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The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b.

ABSTRACT: To detect specific partners of the small Golgi-localized GTPase rab1b we generated rab1b mutants and used them as bait proteins in yeast two-hybrid screens. We isolated several specifically interacting clones. Two of them encode large protein fragments highly homologous to rat GM130 and to human Golgin95. The full-length human GM130 cDNA was cloned and its interaction with rab1b was characterized in detail by yeast two-hybrid and in vitro binding assays. Here we report for the first time that the rab1b protein interacts specifically with GM130 in a GTP-dependent manner and therefore needs the hypervariable regions of the N- and C-termini. We mapped the rab1b binding site of GM130 and provide evidence that it is different to the previously described p115 and Grasp65 binding sites of the GM130 protein.

SUBMITTER: Weide T

PROVIDER: S-EPMC1083862 | biostudies-literature | 2001 Apr

REPOSITORIES: biostudies-literature

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The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b.

Weide T T Bayer M M Köster M M Siebrasse J P JP Peters R R Barnekow A A

EMBO reports 20010401 4

To detect specific partners of the small Golgi-localized GTPase rab1b we generated rab1b mutants and used them as bait proteins in yeast two-hybrid screens. We isolated several specifically interacting clones. Two of them encode large protein fragments highly homologous to rat GM130 and to human Golgin95. The full-length human GM130 cDNA was cloned and its interaction with rab1b was characterized in detail by yeast two-hybrid and in vitro binding assays. Here we report for the first time that th ...[more]

PMID: 11306556

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