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A hybrid bacterial replication origin.

ABSTRACT: We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC.

SUBMITTER: Seitz H

PROVIDER: S-EPMC1084124 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature

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A hybrid bacterial replication origin.

Seitz H H Welzeck M M Messer W W

EMBO reports 20011017 11

We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC. ...[more]

PMID: 11606418

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