Dataset Information

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease.

ABSTRACT: The blood-brain barrier (BBB) is primarily manifested by a variety of physiological properties of brain endothelial cells (ECs), but the molecular foundation for these properties remains incompletely clear. Here, we generate a comprehensive molecular atlas of adult brain ECs using acutely purified mouse ECs and integrated multi-omics. Using RNA sequencing (RNA-seq) and proteomics, we identify the transcripts and proteins selectively enriched in brain ECs and demonstrate that they are partially correlated. Using single-cell RNA-seq, we dissect the molecular basis of functional heterogeneity of brain ECs. Using integrative epigenomics and transcriptomics, we determine that TCF/LEF, SOX, and ETS families are top-ranked transcription factors regulating the BBB. We then validate the identified brain-EC-enriched proteins and transcription factors in normal mouse and human brain tissue and assess their expression changes in mice with Alzheimer's disease. Overall, we present a valuable resource with broad implications for regulation of the BBB and treatment of neurological disorders.

SUBMITTER: Yu M

PROVIDER: S-EPMC10843806 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease.

Yu Min M Nie Yage Y Yang Jiawen J Yang Shilun S Li Rui R Rao Varsha V Hu Xiaoyan X Fang Cheng C Li Simeng S Song Dengpan D Guo Fuyou F Snyder Michael P MP Chang Howard Y HY Kuo Calvin J CJ Xu Jin J Chang Junlei J

Cell reports 20231104 11

The blood-brain barrier (BBB) is primarily manifested by a variety of physiological properties of brain endothelial cells (ECs), but the molecular foundation for these properties remains incompletely clear. Here, we generate a comprehensive molecular atlas of adult brain ECs using acutely purified mouse ECs and integrated multi-omics. Using RNA sequencing (RNA-seq) and proteomics, we identify the transcripts and proteins selectively enriched in brain ECs and demonstrate that they are partially c ...[more]

PMID: 37925638

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Dataset Information

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease.

Publications

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease.

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