Ontology highlight
ABSTRACT:
SUBMITTER: Kim CH
PROVIDER: S-EPMC10858195 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
Kim Chan Hyoung CH Lee Wi-Jae WJ Oh Yeounsun Y Lee Youngjeon Y Lee Hyomin K HK Seong Jung Bae JB Lim Kyung-Seob KS Park Sang Je SJ Huh Jae-Won JW Kim Young-Hyun YH Kim Kyoung Mi KM Hur Junho K JK Lee Seung Hwan SH
Scientific reports 20240209 1
The CRISPR-Cas nickase system for genome editing has attracted considerable attention owing to its safety, efficiency, and versatility. Although alternative effectors to Cas9 have the potential to expand the scope of genome editing, their application has not been optimized. Herein, we used an enhanced CRISPR-Cas12a nickase system to induce mutations by targeting genes in a human-derived cell line. The optimized CRISPR-Cas12a nickase system effectively introduced mutations into target genes under ...[more]