Ontology highlight
ABSTRACT:
SUBMITTER: Huang X
PROVIDER: S-EPMC10859913 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
Huang Xiaofeng X Gu Wei W Zhang Jiaoyue J Lan Ying Y Colarusso Jonathan L JL Li Sanlan S Pertl Christoph C Lu Jiaqi J Kim Hyunkee H Zhu Jian J Breault David T DT Sévigny Jean J Zhou Qiao Q
Nature cell biology 20230427 5
Gut stem cells are accessible by biopsy and propagate robustly in culture, offering an invaluable resource for autologous cell therapies. Insulin-producing cells can be induced in mouse gut, but it has not been possible to generate abundant and durable insulin-secreting cells from human gut tissues to evaluate their potential as a cell therapy for diabetes. Here we describe a protocol to differentiate cultured human gastric stem cells into pancreatic islet-like organoids containing gastric insul ...[more]