Dataset Information

Proposal for standardized ultrasound analysis of the salivary glands: Part 1 submandibular gland.

ABSTRACT:

Objectives

The Salivary Gland Committee of the American Academy of Otolaryngology-Head and Neck Surgery seeks to standardize terminology and technique for ultrasonograpy used in the evaluation and treatment of salivary gland disorders.

Methods

Development of expert opinion obtained through interaction with international practitioners representing multiple specialties. This committee work includes a comprehensive literature review with presentation of case examples to propose a standardized protocol for the language used in ultrasound salivary gland assessment.

Results

A multiple segment proposal is initiated with this focus on the submandibular gland. We provide a concise rationale for recommended descriptive language highlighted by a more extensive supplement that includes an extensive literature review with additional case examples.

Conclusion

Recommendations are provided to improve consistency both in performing and reporting submandibular gland ultrasound.

SUBMITTER: Hoffman HT

PROVIDER: S-EPMC10866606 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Publications

Proposal for standardized ultrasound analysis of the salivary glands: Part 1 submandibular gland.

Hoffman Henry T HT Koch Michael M Witt Robert Lee RL Ryan William R WR Zenk Johannes J Katz Philippe P Rahmati Rahmatullah R Rassekh Christopher C Donato Francisco F McCulloch Timothy M TM Joshi Arjun S AS Chang Jolie Lien JL Gillespie M Boyd MB Pichardo Priscilla F A PFA Orloff Lisa Ann LA Marcelino Antoino A Wenzel Piper P Cohen David D Fundakowski Christopher E CE Cognetti David M DM Walvekar Rohan R RR Bertelli Antonio A Quon Harry H Anderson Carryn C Policeni Bruno B Siegel Gordy G

Laryngoscope investigative otolaryngology 20240214 1

<h4>Objectives</h4>The Salivary Gland Committee of the American Academy of Otolaryngology-Head and Neck Surgery seeks to standardize terminology and technique for ultrasonograpy used in the evaluation and treatment of salivary gland disorders.<h4>Methods</h4>Development of expert opinion obtained through interaction with international practitioners representing multiple specialties. This committee work includes a comprehensive literature review with presentation of case examples to propose a sta ...[more]

PMID: 38362174

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Project description:BackgroundOrgan replacement regenerative therapy based on human adult stem cells may be effective for salivary gland hypofunction. However, the generated tissues are immature because the signaling factors that induce the differentiation of human salivary gland stem cells into salivary glands are unknown.MethodsIsolated human submandibular gland stem/progenitor cells (hSMGepiS/PCs) were characterized and three-dimensionally (3D) cultured to generate organoids and further induced by fibroblast growth factor 10 (FGF10) in vitro. The induced spheres alone or in combination with embryonic day 12.5 (E12.5) mouse salivary gland mesenchyme were transplanted into the renal capsules of nude mice to assess their development in vivo. Immunofluorescence, quantitative reverse transcriptase-polymerase chain reaction, calcium release analysis, western blotting, hematoxylin-eosin staining, Alcian blue-periodic acid-Schiff staining, and Masson's trichrome staining were performed to assess the structure and function of generated tissues in vitro and in vivo.ResultsThe isolated hSMGepiS/PCs could be long-term cultured with a stable genome. The organoids treated with FGF10 [FGF10 (+) group] exhibited higher expression of salivary gland-specific markers; showed spatial arrangement of AQP5+, K19+, and SMA+ cells; and were more sensitive to the stimulation by neurotransmitters than untreated organoids [FGF10 (-) group]. After heterotopic transplantation, the induced cell spheres combined with mouse embryonic salivary gland mesenchyme showed characteristics of mature salivary glands, including a natural morphology and saliva secretion.ConclusionFGF10 promoted the development of the hSMGepiS/PC-derived salivary gland organoids by the expression of differentiation markers, structure formation, and response to neurotransmitters in vitro. Moreover, the hSMGepiS/PCs responded to the niche in mouse embryonic mesenchyme and further differentiated into salivary gland tissues with mature characteristics. Our study provides a foundation for the regenerative therapy of salivary gland diseases.

Dataset Information

Proposal for standardized ultrasound analysis of the salivary glands: Part 1 submandibular gland.

Objectives

Methods

Results

Conclusion

Publications

Proposal for standardized ultrasound analysis of the salivary glands: Part 1 submandibular gland.

Similar Datasets

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