Single-cell RNA sequencing comparison of the human metastatic prostate spine tumor microenvironment.
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ABSTRACT:
SUBMITTER: Nguyen AD
PROVIDER: S-EPMC10867439 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
Publications
Single-cell RNA sequencing comparison of the human metastatic prostate spine tumor microenvironment.
Nguyen Annee D AD Haines Corinne C Price Meghan J MJ Dalton Tara E TE Baëta César D CD Hockenberry Harrison A HA Goodwin C Rory CR
STAR protocols 20240210 1
Spinal column tumors can be difficult to process for single-cell omic studies, given the heterogeneity in tissue. Here, we present a protocol for operating room-to-benchtop single-cell processing of clinical specimens from a prostate cancer patient. We describe steps for sample homogenization, red blood cell lysis, cryopreservation, and single-cell sequencing analysis. This protocol can be used to identify prognostic markers and therapeutic targets for patients with osseous spine metastases and ...[more]
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