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Protocol for monitoring and analyzing pheromone-mediated behavioral response of sea lamprey in a natural system.

ABSTRACT: Olfactory-mediated behaviors in fish are often examined in artificial microcosms that enable well-controlled treatments but fail to replicate environmental and social contexts. However, observing these behaviors in nature poses challenges. Here, we describe a protocol for recording sea lamprey (Petromyzon marinus) behaviors in a natural system. We describe steps for administering and verifying accurate odorant concentrations, surveying sea lamprey abundance, and tracking sea lamprey movements. We also detail procedures to analyze treatment effects on pheromone-mediated spawning in a high-density population. For complete details on the use and execution of this protocol, please refer to Scott et al.¹.

SUBMITTER: Scott AM

PROVIDER: S-EPMC10876582 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Protocol for monitoring and analyzing pheromone-mediated behavioral response of sea lamprey in a natural system.

Scott Anne M AM Johnson Nicholas S NS Siefkes Michael J MJ Li Weiming W

STAR protocols 20240213 1

Olfactory-mediated behaviors in fish are often examined in artificial microcosms that enable well-controlled treatments but fail to replicate environmental and social contexts. However, observing these behaviors in nature poses challenges. Here, we describe a protocol for recording sea lamprey (Petromyzon marinus) behaviors in a natural system. We describe steps for administering and verifying accurate odorant concentrations, surveying sea lamprey abundance, and tracking sea lamprey movements. W ...[more]

PMID: 38358880

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