OsMYB58 Negatively Regulates Plant Growth and Development by Regulating Phosphate Homeostasis
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ABSTRACT:
SUBMITTER: Baek D
PROVIDER: S-EPMC10889527 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
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Project description:Excess Phosphorus (P) in agriculture is causing serious environmental problems like eutrophication of lakes and rivers. Unlike the enormous information available for phosphate starvation response (P0), very few information is available for the effect of excess phosphate Pi on plants. Characterization of Excess Phosphate Response (EPiR) is essential for designing strategies to increase phosphate accumulation and tolerance. We show a significant modulation in the root developmental plasticity under the increasing supply of excess Pi. An excess supply of 20 mM Pi (P20) produces a shallow root system architecture (RSA), reduces primary root growth, root apical meristem size, and meristematic activity in Arabidopsis. The inhibition of primary root growth and development is indeterminate in nature and caused by the decrease in number of meristematic cortical cells due to EPiR. Significant changes occurred in metal nutrients level due to excess Pi supply. A comparative microarray investigation of the EPiR response reveals a modulation in ethylene biosynthesis and signaling, metal ions deficiency response, and root development related genes. We used ethylene-insensitive or sensitive mutants to provide more evidence for ethylene-mediated signaling. A new role of EPiR in regulating the developmental responses of plants mediated by ethylene has been demonstrated.
| S-EPMC5465178 | biostudies-literature
Project description:This SuperSeries is composed of the SubSeries listed below.
2025-12-09 | GSE227771 | GEO
Project description:Although phosphorus is one of the most important essential elements for plant growth and development, the epigenetic regulation of inorganic phosphate (Pi) signaling is poorly understood. In this study, we identified the high-mobility-group protein OsHMGB1 as a key regulator of phosphate homeostasis and plant growth in rice (Oryza sativa). OsHMGB1 expression is induced by Pi starvation and encodes a nucleus-localized protein. Relative to wild-type plants, Oshmgb1 mutant plants had lower Pi content in their leaves, whereas plants overexpressing OsHMGB1 had higher Pi content, indicating that OsHMGB1 positively regulates Pi accumulation. Transcriptome deep sequencing and chromatin immunoprecipitation followed by sequencing showed that OsHMGB1 regulated the expression of a series of phosphate starvation responsive (PSR) genes by binding to their promoters. Furthermore, Assay for Transposase-Accessible Chromatin followed by sequencing revealed that OsHMGB1 was involved in maintaining chromatin accessibility. Indeed, OsHMGB1 occupancy positively correlated with genome-wide chromatin accessibility and gene expression levels. Notably, we determined that OsHMGB1 interacted with RNA polymerase II to help regulate transcription, especially under low Pi conditions. Taken together, our results suggest that OsHMGB1 functions as a transcriptional facilitator, revealing a key epigenetic mechanism to regulate Pi homeostasis and fine-tune plant acclimation responses to Pi-limited environments. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for HMG-GFP transgenic plants
2025-12-09 | GSE227770 | GEO
Project description:Although phosphorus is one of the most important essential elements for plant growth and development, the epigenetic regulation of inorganic phosphate (Pi) signaling is poorly understood. In this study, we identified the high-mobility-group protein OsHMGB1 as a key regulator of phosphate homeostasis and plant growth in rice (Oryza sativa). OsHMGB1 expression is induced by Pi starvation and encodes a nucleus-localized protein. Relative to wild-type plants, Oshmgb1 mutant plants had lower Pi content in their leaves, whereas plants overexpressing OsHMGB1 had higher Pi content, indicating that OsHMGB1 positively regulates Pi accumulation. Transcriptome deep sequencing and chromatin immunoprecipitation followed by sequencing showed that OsHMGB1 regulated the expression of a series of phosphate starvation responsive (PSR) genes by binding to their promoters. Furthermore, Assay for Transposase-Accessible Chromatin followed by sequencing revealed that OsHMGB1 was involved in maintaining chromatin accessibility. Indeed, OsHMGB1 occupancy positively correlated with genome-wide chromatin accessibility and gene expression levels. Notably, we determined that OsHMGB1 interacted with RNA polymerase II to help regulate transcription, especially under low Pi conditions. Taken together, our results suggest that OsHMGB1 functions as a transcriptional facilitator, revealing a key epigenetic mechanism to regulate Pi homeostasis and fine-tune plant acclimation responses to Pi-limited environments.
2025-12-09 | GSE227769 | GEO
Project description:GT factors are trihelix transcription factors that specifically regulate plant development and stress responses. Recently, several GT factors have been characterized in different plant species; however, little is known about the role of GT factors in wheat. Here, we show that TaGT2L1A, TaGT2L1B, and TaGT2L1D are highly homologous in hexaploid wheat, and are localized to wheat chromosomes 2A, 2B, and 2D, respectively. These TaGT2L1 genes encode proteins containing two SANT domains and one central helix. All three homologs were ubiquitously expressed during wheat development and were responsive to osmotic stress. Functional analyses demonstrated that TaGT2L1D acts as a transcriptional repressor; it was able to suppress the expression of AtSDD1 in Arabidopsis by binding directly to the GT3 box in its promoter that negatively regulates drought tolerance. TaGT2L1D overexpression markedly increased the number of stomata and reduced drought tolerance in gtl1-3 plants. Notably, ectopic expression of TaGT2L1D also affected floral organ development and overall plant growth. These results demonstrate that TaGT2L1 is an ortholog of AtGTL1, and that it plays an evolutionarily conserved role in drought resistance by fine tuning stomatal density in wheat. Our data also highlight the role of TaGT2L1 in plant growth and development.
| S-EPMC4887992 | biostudies-literature
Project description:Inorganic phosphate (Pi) is one of the essential molecules for life. However, little is known about intracellular Pi metabolism and signalling in animal tissues1. Following the observation that chronic Pi starvation causes hyperproliferation in the digestive epithelium of Drosophila melanogaster, we determined that Pi starvation triggers the downregulation of the Pi transporter PXo. In line with Pi starvation, PXo deficiency caused midgut hyperproliferation. Interestingly, immunostaining and ultrastructural analyses showed that PXo specifically marks non-canonical multilamellar organelles (PXo bodies). Further, by Pi imaging with a Förster resonance energy transfer (FRET)-based Pi sensor2, we found that PXo restricts cytosolic Pi levels. PXo bodies require PXo for biogenesis and undergo degradation following Pi starvation. Proteomic and lipidomic characterization of PXo bodies unveiled their distinct feature as an intracellular Pi reserve. Therefore, Pi starvation triggers PXo downregulation and PXo body degradation as a compensatory mechanism to increase cytosolic Pi. Finally, we identified connector of kinase to AP-1 (Cka), a component of the STRIPAK complex and JNK signalling3, as the mediator of PXo knockdown- or Pi starvation-induced hyperproliferation. Altogether, our study uncovers PXo bodies as a critical regulator of cytosolic Pi levels and identifies a Pi-dependent PXo-Cka-JNK signalling cascade controlling tissue homeostasis.
| S-EPMC10443203 | biostudies-literature
Project description:Although phosphorus is one of the most important essential elements for plant growth and development, the epigenetic regulation of inorganic phosphate (Pi) signaling is poorly understood. In this study, we identified the high-mobility-group protein OsHMGB1 as a key regulator of phosphate homeostasis and plant growth in rice (Oryza sativa). OsHMGB1 expression is induced by Pi starvation and encodes a nucleus-localized protein. Relative to wild-type plants, Oshmgb1 mutant plants had lower Pi content in their leaves, whereas plants overexpressing OsHMGB1 had higher Pi content, indicating that OsHMGB1 positively regulates Pi accumulation. Transcriptome deep sequencing and chromatin immunoprecipitation followed by sequencing showed that OsHMGB1 regulated the expression of a series of phosphate starvation responsive (PSR) genes by binding to their promoters. Furthermore, Assay for Transposase-Accessible Chromatin followed by sequencing revealed that OsHMGB1 was involved in maintaining chromatin accessibility. Indeed, OsHMGB1 occupancy positively correlated with genome-wide chromatin accessibility and gene expression levels. Notably, we determined that OsHMGB1 interacted with RNA polymerase II to help regulate transcription, especially under low Pi conditions. Taken together, our results suggest that OsHMGB1 functions as a transcriptional facilitator, revealing a key epigenetic mechanism to regulate Pi homeostasis and fine-tune plant acclimation responses to Pi-limited environments.
2025-12-09 | GSE227768 | GEO
Project description:Although phosphorus is one of the most important essential elements for plant growth and development, the epigenetic regulation of inorganic phosphate (Pi) signaling is poorly understood. In this study, we identified the high-mobility-group protein OsHMGB1 as a key regulator of phosphate homeostasis and plant growth in rice (Oryza sativa). OsHMGB1 expression is induced by Pi starvation and encodes a nucleus-localized protein. Relative to wild-type plants, Oshmgb1 mutant plants had lower Pi content in their leaves, whereas plants overexpressing OsHMGB1 had higher Pi content, indicating that OsHMGB1 positively regulates Pi accumulation. Transcriptome deep sequencing and chromatin immunoprecipitation followed by sequencing showed that OsHMGB1 regulated the expression of a series of phosphate starvation responsive (PSR) genes by binding to their promoters. Furthermore, Assay for Transposase-Accessible Chromatin followed by sequencing revealed that OsHMGB1 was involved in maintaining chromatin accessibility. Indeed, OsHMGB1 occupancy positively correlated with genome-wide chromatin accessibility and gene expression levels. Notably, we determined that OsHMGB1 interacted with RNA polymerase II to help regulate transcription, especially under low Pi conditions. Taken together, our results suggest that OsHMGB1 functions as a transcriptional facilitator, revealing a key epigenetic mechanism to regulate Pi homeostasis and fine-tune plant acclimation responses to Pi-limited environments.
2025-12-09 | GSE227767 | GEO
Project description:Glycine- and proline-rich proteins (GPRPs) comprise a small conserved family that is widely distributed in the plant kingdom. GPRPs are relatively short peptides (<200 amino acids) that contain three typical domains, including an N-terminal XYPP-repeat domain, a middle hydrophobic domain rich in alanine, and a C-terminal HGK-repeat domain. These proteins have been proposed to play fundamental roles in plant growth and environmental adaptation, but their functions remain unknown. In this study, we selected an Arabidopsis GPRP (AtGPRP3) to profile the physiological role of GPRPs. Transcripts of AtGPRP3 could be detected in the whole Arabidopsis plant, but greater amounts were found in the rosette, followed by the cauline. The AtGPRP3::GFP fusion protein was mainly localized in the nucleus. The overexpression and knockout of AtGPRP3, respectively, retarded and accelerated the growth of Arabidopsis seedlings, while the increase in the growth rate of atgprp3 plants was offset by the complementary expression of AtGPRP3. CAT2 and CAT3, but not CAT1, interacted with AtGPRP3 in the nuclei of Arabidopsis protoplasts. The knockout of CAT2 by CRISPR-Cas9 retarded the growth of the Arabidopsis seedlings. Together, our data suggest that AtGPRP3 negatively regulates plant growth, potentially through CAT2 and CAT3.
| S-EPMC7504531 | biostudies-literature
Project description:Internode elongation is one of the key agronomic traits determining a plant's height and biomass. However, our understanding of the molecular mechanisms controlling internode elongation is still limited in crop plant species. Here, we report the functional identification of an atypical basic helix-loop-helix transcription factor (OsbHLH073) through gain-of-function studies using overexpression (OsbHLH073-OX) and activation tagging (osbhlh073-D) lines of rice. The expression of OsbHLH073 was significantly increased in the osbhlh073-D line. The phenotype of osbhlh073-D showed semi-dwarfism due to deficient elongation of the first internode and poor panicle exsertion. Transgenic lines overexpressing OsbHLH073 confirmed the phenotype of the osbhlh073-D line. Exogenous gibberellic acid (GA3) treatment recovered the semi-dwarf phenotype of osbhlh073-D plants at the seedling stage. In addition, quantitative expression analysis of genes involving in GA biosynthetic and signaling pathway revealed that the transcripts of rice ent-kaurene oxidases 1 and 2 (OsKO1 and OsKO2) encoding the GA biosynthetic enzyme were significantly downregulated in osbhlh073-D and OsbHLH073-OX lines. Yeast two-hybrid and localization assays showed that the OsbHLH073 protein is a nuclear localized-transcriptional activator. We report that OsbHLH073 participates in regulating plant height, internode elongation, and panicle exsertion by regulating GA biosynthesis associated with the OsKO1 and OsKO2 genes.
| S-EPMC7238965 | biostudies-literature