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Sensitivity of lateral flow technique for diagnosis of canine parvovirus.

ABSTRACT: In this study, we devised a nanogold lateral flow immunoassay (LFA-CPV antigen test) for detecting canine parvovirus (CPV) in living attenuated CPV vaccines. We conducted instrumental characterization of the prepared nanogold particles and the developed LFA-CPV antigen test was rigorously evaluated for its performance verification including limit of detection, sensitivity, specificity, selectivity and accuracy. The LFA-CPV antigen test demonstrated strong performance when assessed against qPCR using different batches of live attenuated CPV vaccines, indicated a sensitivity of 96.4%, specificity of 88.2%, and an overall accuracy of 95%. These results suggest that the developed LFA-CPV antigen test could serve as a viable alternative for evaluation live attenuated CPV vaccines, and provide it as a point of care test for CPV diagnosis, offering a potential substitute for traditional laboratory methods, particularly qPCR.

SUBMITTER: Abousenna MS

PROVIDER: S-EPMC10904390 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Sensitivity of lateral flow technique for diagnosis of canine parvovirus.

Abousenna M S MS Sayed R H RH E Shaimaa A SA Shasha F A FA El Sawy Sara E A SEA Darwish D M DM

Scientific reports 20240301 1

In this study, we devised a nanogold lateral flow immunoassay (LFA-CPV antigen test) for detecting canine parvovirus (CPV) in living attenuated CPV vaccines. We conducted instrumental characterization of the prepared nanogold particles and the developed LFA-CPV antigen test was rigorously evaluated for its performance verification including limit of detection, sensitivity, specificity, selectivity and accuracy. The LFA-CPV antigen test demonstrated strong performance when assessed against qPCR u ...[more]

PMID: 38424259

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