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Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.

ABSTRACT: mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3' end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3'-5' mRNA degradation intermediates in yeast.

SUBMITTER: Boeck R

PROVIDER: S-EPMC109091 | biostudies-literature | 1998 Sep

REPOSITORIES: biostudies-literature

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Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.

Boeck R R Lapeyre B B Brown C E CE Sachs A B AB

Molecular and cellular biology 19980901 9

mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detectio ...[more]

PMID: 9710590

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