Genomic and virulence analysis of in vitro cultured Cryptosporidium parvum.
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ABSTRACT:
SUBMITTER: Yarlett N
PROVIDER: S-EPMC10927135 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
Publications
Genomic and virulence analysis of in vitro cultured Cryptosporidium parvum.
Yarlett Nigel N Morada Mary M Schaefer Deborah A DA Ackman Kevin K Carranza Elizabeth E Baptista Rodrigo de Paula RP Riggs Michael W MW Kissinger Jessica C JC
PLoS pathogens 20240228 2
Recent advances in the in vitro cultivation of Cryptosporidium parvum using hollow fiber bioreactor technology (HFB) have permitted continuous growth of parasites that complete all life cycle stages. The method provides access to all stages of the parasite and provides a method for non-animal production of oocysts for use in clinical trials. Here we examined the effect of long-term (>20 months) in vitro culture on virulence-factors, genome conservation, and in vivo pathogenicity of the host by i ...[more]
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