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Preserving the adaptive salt stress response activity of a tissue-specific promoter with modulating activity.

ABSTRACT:

Background

The Arabidopsis "Redox Responsive Transcription Factor1" (RRTF1) promoter is transiently activated by salt stress in roots over 6 h period, followed by an adaptation phase during which its activity returns to baseline levels, even if the salt stress is prolonged. This enables the short-term production of genes that, while initially advantageous to the plant, will have long-term detrimental effects if expressed at high levels indefinitely.

Results

In this paper, we demonstrate that the RRTF1 promoter salt adaption response is a dominant feature of the promoter, that cannot be overwritten by a strong enhancer. While maintaining the transient activation profile of the RRTF1 promoter, linking it to the 35S enhancer results in a significant boost of salt stress induction in roots.

Conclusion

The RRTF1 promoter's enhanced and still adaptable activity could become a useful tool in plant biotechnology.

SUBMITTER: Soliman ERS

PROVIDER: S-EPMC10941203 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Preserving the adaptive salt stress response activity of a tissue-specific promoter with modulating activity.

Soliman Elham R S ERS

Journal, genetic engineering & biotechnology 20240201 1

<h4>Background</h4>The Arabidopsis "Redox Responsive Transcription Factor1" (RRTF1) promoter is transiently activated by salt stress in roots over 6 h period, followed by an adaptation phase during which its activity returns to baseline levels, even if the salt stress is prolonged. This enables the short-term production of genes that, while initially advantageous to the plant, will have long-term detrimental effects if expressed at high levels indefinitely.<h4>Results</h4>In this paper, we demon ...[more]

PMID: 38494266

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