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Loss of the E3 ubiquitin ligase TRIM67 alters the post-synaptic density proteome.

ABSTRACT: The E3 ubiquitin ligase TRIM67 is enriched in the central nervous system and is required for proper neuronal development. Previously we demonstrated TRIM67 coordinates with the closely related E3 ubiquitin ligase TRIM9 to regulate cytoskeletal dynamics downstream of the netrin-1 during axon guidance and axon branching in early neuronal morphogenesis. Interestingly, loss of Trim67 impacts cognitive flexibility in a spatial learning and memory task. Despite this behavioral phenotype, it was previously uninvestigated if TRIM67 was involved in synapse formation or function. Here we demonstrate TRIM67 localizes to the post-synaptic density (PSD) within dendritic spines. Furthermore, we show that loss of Trim67 significantly changes a subset of proteins within the PSD proteome, including changes in the regulation of the actin and microtubule cytoskeletons. Collectively, our data propose a synaptic role for TRIM67.

SUBMITTER: Mccormick LE

PROVIDER: S-EPMC10943362 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature

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Loss of the E3 ubiquitin ligase TRIM67 alters the post-synaptic density proteome.

Mccormick Laura E LE Baker Natalie K NK Herring Laura E LE Gupton Stephanie L SL

microPublication biology 20240301

The E3 ubiquitin ligase TRIM67 is enriched in the central nervous system and is required for proper neuronal development. Previously we demonstrated TRIM67 coordinates with the closely related E3 ubiquitin ligase TRIM9 to regulate cytoskeletal dynamics downstream of the netrin-1 during axon guidance and axon branching in early neuronal morphogenesis. Interestingly, loss of <i>Trim67</i> impacts cognitive flexibility in a spatial learning and memory task. Despite this behavioral phenotype, it was ...[more]

PMID: 38495584

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Project description:During development, neurons undergo expansive growth and shape change to achieve their mature morphology and physiological function. As cellular remodeling occurs, proteins are synthesized, transported, degraded, and recycled. As such, protein levels and localization are tightly regulated. E3 ubiquitin ligases play a key role in proteostasis by altering protein localization, intracellular trafficking, and protein lifetime via the addition of ubiquitin modifiers. TRIM9 and TRIM67 are brain-enriched E3 ubiquitin ligases implicated in numerous stages of neuronal morphogenesis. We previously demonstrated both proteins are required for axon pathfinding and growth cone shape changes in developing neurons. In particular, TRIM9 and TRIM67 regulate filopodia number and stability in early stages of neuronal development. Our published in vivo studies suggest TRIM9 and TRIM67 may function at the synapse as well. We observed distinct deficits in spatial learning and memory of Trim9-/- and Trim67-/- mice in the Morris Water Maze test, compared to their littermate controls. Furthermore, adult-born neurons in the dentate gyrus in Trim9-/- mice also displayed a decreased number of dendritic spines. Here we demonstrate TRIM9 and TRIM67 localize to the post-synaptic density (PSD), a structure attached to the post-synaptic membrane in dendritic spines. We identified 148 proteins that were significantly changed (p < 0.05) in the Trim67-/- mice compared to their Trim67+/+ littermates. Gene Ontology analysis of these proteins demonstrated enrichment of several cellular pathways, including peptidyl-amino acid modification, microtubule dynamics, and Ras signal transduction. Likewise, we identified 109 proteins that were significantly changed (p < 0.05) in the Trim9-/- mice compared to their Trim9+/+ littermates. Following Gene Ontology analysis, we observed the prominent enrichment of one pathway in our significantly different proteins: the actin cytoskeleton. Changes in these actin cytoskeleton proteins were bidirectional, suggesting the presence of altered cytoskeletal architecture and organization in the Trim9-/- PSD.

Dataset Information

Loss of the E3 ubiquitin ligase TRIM67 alters the post-synaptic density proteome.

Publications

Loss of the E3 ubiquitin ligase TRIM67 alters the post-synaptic density proteome.

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