Dataset Information

Effect of urine alkalization on urinary inflammatory markers in cystinuric patients.

ABSTRACT:

Background

Cystinuria is associated with a high prevalence of chronic kidney disease (CKD). We previously described a urinary inflammatory-protein signature (UIS), including 38 upregulated proteins, in cystinuric patients (Cys-patients), compared with healthy controls (HC). This UIS was higher in Cys-patients with CKD. In the present observational study, we aimed to investigate the UIS in Cys-patients without CKD and patients with calcium nephrolithiasis (Lith-patients), versus HC and the effect of urine alkalization on the UIS of Cys-patients.

Methods

UIS was evaluated by nano-liquid chromatography coupled to high-resolution mass spectrometry in adult HC, Lith-patients and non-treated Cys-patients with an estimated glomerular filtration rate >60 mL/min/1.73 m², and after a 3-month conventional alkalizing treatment in Cys-patients.

Results

Twenty-one Cys-patients [12 men, median age (interquartile range) 30.0 (25.0-44.0) years], 12 Lith-patients [8 men, 46.2 (39.5-54.2) years] and 7 HC [2 men, 43.1 (31.0-53.9) years] were included. Among the 38 proteins upregulated in our previous work, 11 proteins were also upregulated in Cys-patients compared with HC in this study (5 circulating inflammatory proteins and 6 neutrophil-derived proteins). This UIS was also found in some Lith-patients. Using this UIS, we identified two subclusters of Cys-patients (5 with a very high/high UIS and 16 with a moderate/low UIS). In the Cys-patients with very high/high UIS, urine alkalization induced a significant decrease in urinary neutrophil-derived proteins.

Conclusion

A high UIS is present in some Cys-patients without CKD and decreases under alkalizing treatment. This UIS could be a prognostic marker to predict the evolution towards CKD in cystinuria.

SUBMITTER: Prot-Bertoye C

PROVIDER: S-EPMC10953617 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Effect of urine alkalization on urinary inflammatory markers in cystinuric patients.

Prot-Bertoye Caroline C Jung Vincent V Tostivint Isabelle I Roger Kevin K Benoist Jean-François JF Jannot Anne-Sophie AS Van Straaten Alexis A Knebelmann Bertrand B Guerrera Ida Chiara IC Courbebaisse Marie M

Clinical kidney journal 20240222 3

<h4>Background</h4>Cystinuria is associated with a high prevalence of chronic kidney disease (CKD). We previously described a urinary inflammatory-protein signature (UIS), including 38 upregulated proteins, in cystinuric patients (Cys-patients), compared with healthy controls (HC). This UIS was higher in Cys-patients with CKD. In the present observational study, we aimed to investigate the UIS in Cys-patients without CKD and patients with calcium nephrolithiasis (Lith-patients), versus HC and th ...[more]

PMID: 38510798

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Dataset Information

Effect of urine alkalization on urinary inflammatory markers in cystinuric patients.

Background

Methods

Results

Conclusion

Publications

Effect of urine alkalization on urinary inflammatory markers in cystinuric patients.

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