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Protocol for fast clonal family inference and analysis from large-scale B cell receptor repertoire sequencing data.

ABSTRACT: The expeditious identification and comprehensive analysis of clonal families from extensive B cell receptor (BCR) repertoire sequencing data are imperative for elucidating the intricacies of B cell immune responses. Here, we introduce a computational pipeline designed to swiftly deduce clonal families from bulk BCR heavy-chain sequencing data, accompanied by a suite of functional modules tailored to streamline post-clustering analysis. The outlined methodology encompasses guidelines for software installation, meticulous data preparation, and the systematic inference and analysis of clonal families. For complete details on the use and execution of this protocol, please refer to Wang et al.¹.

SUBMITTER: Wang K

PROVIDER: S-EPMC10963638 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Protocol for fast clonal family inference and analysis from large-scale B cell receptor repertoire sequencing data.

Wang Kaixuan K Cai Linru L Wang Hao H Shan Shiwen S Hu Xihao X Zhang Jian J

STAR protocols 20240318 2

The expeditious identification and comprehensive analysis of clonal families from extensive B cell receptor (BCR) repertoire sequencing data are imperative for elucidating the intricacies of B cell immune responses. Here, we introduce a computational pipeline designed to swiftly deduce clonal families from bulk BCR heavy-chain sequencing data, accompanied by a suite of functional modules tailored to streamline post-clustering analysis. The outlined methodology encompasses guidelines for software ...[more]

PMID: 38502687

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