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Tunable fluorescent probes for detecting aldehydes in living systems.

ABSTRACT: Aldehydes, pervasive in various environments, pose health risks at elevated levels due to their collective toxic effects via shared mechanisms. Monitoring total aldehyde content in living systems is crucial due to their cumulative impact. Current methods for detecting cellular aldehydes are limited to UV and visible ranges, restricting their analysis in living systems. This study introduces an innovative reaction-based trigger that leverages the exceptional selectivity of 2-aminothiophenol for aldehydes, leading to the production of dihydrobenzothiazole and activating a fluorescence response. Using this trigger, we developed a series of fluorescent probes for aldehydes by altering the fluorophore allowing for excitation and emission wavelengths across the visible to near-infrared spectral regions without compromising the reactivity of the bioorthogonal moiety. These probes exhibit remarkable aldehyde chemoselectivity, rapid kinetics, and high quantum yields, enabling the detection of diverse aldehyde types, both exogenous and endogenous, within complex biological contexts. Notably, we employed the most red-shifted near-infrared probe from this series to detect aldehydes in living systems, including biliary organoids and mouse organs. These probes provide valuable tools for exploring the multifaceted roles of aldehydes in biological functions and diseases within living systems, laying the groundwork for further investigations.

SUBMITTER: Wills R

PROVIDER: S-EPMC10966992 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Tunable fluorescent probes for detecting aldehydes in living systems.

Wills Rachel R Shirke Rajendra R Hrncir Hannah H Talbott John M JM Sad Kirti K Spangle Jennifer M JM Gracz Adam D AD Raj Monika M

Chemical science 20240319 13

Aldehydes, pervasive in various environments, pose health risks at elevated levels due to their collective toxic effects <i>via</i> shared mechanisms. Monitoring total aldehyde content in living systems is crucial due to their cumulative impact. Current methods for detecting cellular aldehydes are limited to UV and visible ranges, restricting their analysis in living systems. This study introduces an innovative reaction-based trigger that leverages the exceptional selectivity of 2-aminothiopheno ...[more]

PMID: 38550703

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Project description:Retractions are becoming increasingly common but still account for a small minority of published papers. It would be useful to generate databases where the presence of retractions can be linked to impact metrics of each scientist. We have thus incorporated retraction data in an updated Scopus-based database of highly cited scientists (top 2% in each scientific subfield according to a composite citation indicator). Using data from the Retraction Watch database (RWDB), retraction records were linked to Scopus citation data. Of 55,237 items in RWDB as of August 15, 2024, we excluded non-retractions, retractions clearly not due to any author error, retractions where the paper had been republished, and items not linkable to Scopus records. Eventually, 39,468 eligible retractions were linked to Scopus. Among 217,097 top-cited scientists in career-long impact and 223,152 in single recent year (2023) impact, 7,083 (3.3%) and 8,747 (4.0%), respectively, had at least 1 retraction. Scientists with retracted publications had younger publication age, higher self-citation rates, and larger publication volume than those without any retracted publications. Retractions were more common in the life sciences and rare or nonexistent in several other disciplines. In several developing countries, very high proportions of top-cited scientists had retractions (highest in Senegal (66.7%), Ecuador (28.6%), and Pakistan (27.8%) in career-long citation impact lists). Variability in retraction rates across fields and countries suggests differences in research practices, scrutiny, and ease of retraction. Addition of retraction data enhances the granularity of top-cited scientists' profiles, aiding in responsible research evaluation. However, caution is needed when interpreting retractions, as they do not always signify misconduct; further analysis on a case-by-case basis is essential. The database should hopefully provide a resource for meta-research and deeper insights into scientific practices.

Dataset Information

Tunable fluorescent probes for detecting aldehydes in living systems.

Publications

Tunable fluorescent probes for detecting aldehydes in living systems.

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