Dataset Information

Detection of Intestinal Inflammation by Vascular Adhesion Protein-1-Targeted [⁶⁸Ga]Ga-DOTA-Siglec-9 Positron Emission Tomography in Murine Models of Inflammatory Bowel Disease.

ABSTRACT:

Purpose

Inflammatory bowel disease (IBD) can be imaged with positron emission tomography (PET), but existing PET radiopharmaceuticals have limited diagnostic accuracy. Vascular adhesion protein-1 (VAP-1) is an endothelial cell surface molecule that controls leukocyte extravasation into sites of inflammation. However, the role of inflammation-induced VAP-1 expression in IBD is still unclear. Therefore, this study investigated the utility of VAP-1-targeted [⁶⁸Ga]Ga-DOTA-Siglec-9 positron emission tomography/computed tomography (PET/CT) for assessing inflammation in two mouse models of IBD.

Procedures

Studies were performed using K8^-/- mice that develop a chronic colitis-phenotype and C57Bl/6NCrl mice with acute intestinal inflammation chemically-induced using 2.5% dextran sodium sulfate (DSS) in drinking water. In both diseased and control mice, uptake of the VAP-1-targeting peptide [⁶⁸Ga]Ga-DOTA-Siglec-9 was assessed in intestinal regions of interest using in vivo PET/CT, after which ex vivo gamma counting, digital autoradiography, and histopathological analyses were performed. Immunofluorescence staining was performed to determine VAP-1-expression in the intestine, including in samples from patients with ulcerative colitis.

Results

Intestinal inflammation could be visualized by [⁶⁸Ga]Ga-DOTA-Siglec-9 PET/CT in two murine models of IBD. In both models, the in vivo PET/CT and ex vivo studies of [⁶⁸Ga]Ga-DOTA-Siglec-9 uptake were significantly higher than in control mice. The in vivo uptake was increased on average 1.4-fold in the DSS model and 2.0-fold in the K8^-/- model. Immunofluorescence staining revealed strong expression of VAP-1 in the inflamed intestines of both mice and patients.

Conclusions

This study suggests that the VAP-1-targeting [⁶⁸Ga]Ga-DOTA-Siglec-9 PET tracer is a promising tool for non-invasive imaging of intestinal inflammation. Future studies in patients with IBD and evaluation of the potential value of [⁶⁸Ga]Ga-DOTA-Siglec-9 in diagnosis and monitoring of the disease are warranted.

SUBMITTER: Bhowmik AA

PROVIDER: S-EPMC10973022 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature

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Publications

Detection of Intestinal Inflammation by Vascular Adhesion Protein-1-Targeted [<sup>68</sup>Ga]Ga-DOTA-Siglec-9 Positron Emission Tomography in Murine Models of Inflammatory Bowel Disease.

Bhowmik Achol A AA Heikkilä Taina R H TRH Polari Lauri L Virta Jenni J Liljenbäck Heidi H Moisio Olli O Li Xiang-Guo XG Viitanen Riikka R Jalkanen Sirpa S Koffert Jukka J Toivola Diana M DM Roivainen Anne A

Molecular imaging and biology 20231218 2

<h4>Purpose</h4>Inflammatory bowel disease (IBD) can be imaged with positron emission tomography (PET), but existing PET radiopharmaceuticals have limited diagnostic accuracy. Vascular adhesion protein-1 (VAP-1) is an endothelial cell surface molecule that controls leukocyte extravasation into sites of inflammation. However, the role of inflammation-induced VAP-1 expression in IBD is still unclear. Therefore, this study investigated the utility of VAP-1-targeted [<sup>68</sup>Ga]Ga-DOTA-Siglec-9 ...[more]

PMID: 38110791

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Dataset Information

Detection of Intestinal Inflammation by Vascular Adhesion Protein-1-Targeted [⁶⁸Ga]Ga-DOTA-Siglec-9 Positron Emission Tomography in Murine Models of Inflammatory Bowel Disease.

Purpose

Procedures

Results

Conclusions

Publications

Detection of Intestinal Inflammation by Vascular Adhesion Protein-1-Targeted [<sup>68</sup>Ga]Ga-DOTA-Siglec-9 Positron Emission Tomography in Murine Models of Inflammatory Bowel Disease.

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OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Detection of Intestinal Inflammation by Vascular Adhesion Protein-1-Targeted [68Ga]Ga-DOTA-Siglec-9 Positron Emission Tomography in Murine Models of Inflammatory Bowel Disease.

Purpose

Procedures

Results

Conclusions

Publications

Detection of Intestinal Inflammation by Vascular Adhesion Protein-1-Targeted [&lt;sup&gt;68&lt;/sup&gt;Ga]Ga-DOTA-Siglec-9 Positron Emission Tomography in Murine Models of Inflammatory Bowel Disease.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Detection of Intestinal Inflammation by Vascular Adhesion Protein-1-Targeted [⁶⁸Ga]Ga-DOTA-Siglec-9 Positron Emission Tomography in Murine Models of Inflammatory Bowel Disease.

Detection of Intestinal Inflammation by Vascular Adhesion Protein-1-Targeted [<sup>68</sup>Ga]Ga-DOTA-Siglec-9 Positron Emission Tomography in Murine Models of Inflammatory Bowel Disease.