Economic and environmental assessment of bacterial poly(3-hydroxybutyrate) production from the organic fraction of municipal solid waste
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ABSTRACT:
SUBMITTER: Izaguirre J
PROVIDER: S-EPMC10992733 | biostudies-literature | 2021 May
REPOSITORIES: biostudies-literature
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Project description:Mechanical-biological treatment of municipal solid waste (MSW) facilitates reducing the landfill workload. The current research aimed to study general activity parameters, content, functions, and diversity of fungal and prokaryotic microbiota in mechanically separated organic fraction of MSW (ms-OFMSW) composting, without using bulking agents and process-promoting additives. During 35 days of composting, vigorous emission of CO2 (max. 129.4 mg CO2 kg-1 h-1), NH3 (max. 0.245 mg NH3 kg-1 h-1), and heat release (max. 4.28 kJ kg-1 h-1) occurred, indicating intense microbial activity. Immediately following the preparation of the composting mixture, eight genera of lactic acid bacteria and fungal genera Rhizopus, Aspergillus, Penicillium, Agaricus, and Candida were predominant. When the temperature increased to more than 60 °C, the microbial biodiversity decreased. Due to succession, the main decomposers of ms-OFMSW changed. The Bacillaceae family, the genera Planifilum, Thermobifida, and Streptomyces, and the fungal genera Thermomyces and Microascus were involved in the processes of organic matter mineralization at the high-temperature and later stages. The biodiversity of the microbiota increased at the stages of cooling and maturation under conditions of relatively high nitrogen content. Thus, the microbial community and its succession during ms-OFMSW composting were characterized for the first time in this work.
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Project description:The increase in municipal solid waste (MSW) generation rate has been a growing concern for the modern-day era. On-site composting has been the promising clean-tech alternative to managing biodegradable organic waste (BOW) in MSW. It allows sustainable and compact solutions for the in-house treatment of MSW, reducing the overall burden on landfill and treatment facilities. In this manuscript, a batch and pilot scale performance assessment study were conducted for BOW using a three-stage vertical drum composter (R1, R2, R3). The study aims to determine the impact of aeration, turning mechanisms, bulking agents, degradation rate, and process parameters on compost quality. It was found that physical-chemical properties such as bulk density (0.3 g/cm3), pH (∼7), temperature (<50 °C), moisture content (<20 %), total volatile solids (33 %), electrical conductivity (<4 dS/m) and carbon/nitrogen ratio (∼16) of final compost was under the prescribed limit. We conclude that the provision for aeration via perforated vents and regular turning mechanisms substantially impacted the quality of compost. Compost maturity was determined using humic to fulvic acid (HA/FA) ratio and germination index (GI). The HA/FA and GI of final compost in R1, R2, and R3 were found to be 6.21, 7.22, and 6.90; and 85.3 %, 90.4 %, and 87.6 %, respectively. During the degradation process, the increasing trend of HA/FA ratio (5-8) and GI (>85 %) showed that the compost quality was rich in nutrients and soil-conditioning properties. Based on the kinetic study, it was conclusive that adding bulking agents in R3 (0.0078 day-1) and R4 (0.0098 day-1) contributed to high degradation rates, underlining the value of creating a porous structure that enhances microbial activity. The findings can be a resource for waste generators, managers, technocrats, and policymakers to tackle the issues related to in-house management and treatment of MSW.
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Project description:The utilisation of the organic fraction of municipal solid waste as feedstock for bioethanol production could reduce the need for disposal of the ever-increasing amounts of municipal solid waste, especially in developing countries, and fits with the integrated goals of climate change mitigation and transport energy security. Mixed culture fermentation represents a suitable approach to handle the complexity and variability of such waste, avoiding expensive and vulnerable closed-control operational conditions. It is widely accepted that the control of pH in these systems can direct the fermentation process toward a desired fermentation product, however, little empirical evidence has been provided in respect of lignocellulosic waste substrates and different environmental inocula sources. We evaluated ethanol production from the organic fraction of municipal solid waste using five different inocula sources where lignocellulose degradation putatively occurs, namely, compost, woodland soil, rumen, cow faeces and anaerobic granular sludge, when incubated in batch microcosms at either initially neutral or acidic pH and under initially aerobic or anaerobic conditions. Although ethanol was produced by all the inocula tested, their performance was different in response to the imposed experimental conditions. Rumen and anaerobic granular sludge produced significantly the highest ethanol concentrations (∼30 mM) under initially neutral and acidic pH, respectively. A mixed-source community formed by mixing rumen and sludge (R + S) was then tested over a range of initial pH. In contrast to the differences observed for the individual inocula, the maximal ethanol production of the mixed community was not significantly different at initial pH of 5.5 and 7. Consistent with this broader functionality, the microbial community analyses confirmed the R + S community enriched comprised bacterial taxa representative of both original inocula. It was demonstrated that the interaction of initial pH and inocula source dictated ethanologenic activity from the organic fraction of municipal solid waste. Furthermore, the ethanologenic mixed-source community enriched, was comprised of taxa belonging to the two original inocula sources (rumen and sludge) and had a broader functionality. This information is relevant when diverse inocula sources are combined for mix culture fermentation studies as it experimentally demonstrates the benefits of diversity and function assembled from different inocula.
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Project description:The presented dataset in this data article provides quantitative data on the production of bioenergy (biogas and biomethane) from mesophilic batch anaerobic digestion (AD) of thermally hydrolyzed organic fraction of municipal solid waste (OFMSW). The discussion and interpretation of the data are provided in another publication entitled "Hydrothermal Pretreatment of Source Separated Organics for Enhanced Solubilization and Biomethane Recovery" (Razavi et al., 2019). The data and information presented in the current data article include (1) the ratio of soluble to particulate chemical oxygen demand (COD) under different thermal hydrolysis condition, (2) the daily measured biogas and biomethane data, (3) the cumulative methane yield data in terms of mL CH4 produced per gram of volatile suspended solids (VSS) as well as feedstock added, (4) the ultimate methane yield data as well as the relative improvement in methane recovery compared to the control (non-hydrolyzed) digester, (5) the data of first-order organics biodegradation rate constants, (6) the procedure of measuring biogas composition via gas chromatography, (7) the procedure of converting the biogas/methane volume data acquired under the actual experimental condition (mesophilic temperature of 38 °C and atmospheric pressure) to the standard temperature (0 °C) and pressure (1 atm) condition, and (8) the procedure of determining the first-order kinetic rate constants.
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Project description:While bioplastics are gaining wide interest in replacing conventional plastics, it is necessary to understand whether the treatment of the organic fraction of municipal solid waste (OFMSW) as an end-of-life option is compatible with their biodegradation and their possible role in shaping the microbial communities involved in the processes. In the present work, we assessed the microbiological impact of rigid polylactic acid (PLA) and starch-based bioplastics (SBB) spoons on the thermophilic anaerobic digestion and the aerobic composting of OFMSW under real plant conditions. In order to thoroughly evaluate the effect of PLA and SBB on the bacterial, archaeal, and fungal communities during the process, high-throughput sequencing (HTS) technology was carried out. The results suggest that bioplastics shape the communities' structure, especially in the aerobic phase. Distinctive bacterial and fungal sequences were found for SBB compared to the positive control, which showed a more limited diversity. Mucor racemosus was especially abundant in composts from bioplastics' treatment, whereas Penicillium roqueforti was found only in compost from PLA and Thermomyces lanuginosus in that from SBB. This work shed a light on the microbial communities involved in the OFMSW treatment with and without the presence of bioplastics, using a new approach to evaluate this end-of-life option.
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Project description:The organic fraction of municipal solid waste (OFMSW) is a complex material with different ingredients characterized by varying properties depending on parameters such as season or geographical region of origin. Consequently, studies on OFMSW are hard to compare due to the changing characteristics of the samples. Therefore, this article presents data on the physico-chemical composition of standardized, recipe-based OFMSW components divided into the categories "Paper", "Green waste" and "Food waste", and further subcategories. Data presented in this article include (1) dry matter, (2) organic dry matter, (3) C, H and N concentrations, (4) gross calorific values, (5) ash melting behavior, (6) specific biogas yield and (7) methane concentration. An application example of an experiment requiring the same starting material properties is represented by storage experiments, as performed within the original scientific article [1]. Thus, this Data in Brief article also provides additional data on recipe-based storage experiments complementing the original article. The datasets cannot only be used to estimate biowaste potentials but they can also be used for the design and execution of experiments that require standardized OFMSW samples.
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Project description:This study is focused on the effects of pH on the production of volatile fatty acids (VFAs) and their distribution through the acidogenic fermentation of source-sorted organic fraction of municipal solid waste (OFMSW) from a mechanical-biological treatment (MBT) plant, and food waste (FW) from a university canteen. In semi-continuous lab-scale digesters using OFMSW at a hydraulic retention time (HRT) of 3.5 days under acidic conditions (pH 6.0), the VFA concentration in the effluent increased to 9.8-11.5 g L-1 (VS content of the feedstock between 4.2 and 5.2% w/w), while its individual VFA profiling was similar to the influent which was already pre-fermented (namely, C2 35-41%, C3 18-22%, C4 17-21%, and C5 9-12%). When working with the same conditions but using FW as feedstock, an effluent with a VFA concentration up to 11.5 g VFA L-1 (FW with a VS content of 5.5% w/w) and a stable distribution of C2 and C4 acids (up to 60.3% and 12.9%, respectively) but with very low quantities of C3 and C5 acids (lower than 1.8 and 2.7%, respectively) was obtained. Anaerobic batch tests using FW revealed that alkaline pH near 9 could lead to higher VFA production with high acetic acid content when compared to pH 6. In the semi-continuous fermenters working at alkaline conditions (pH 9.5-10) using OFMSW and FW, an enhanced solubilization of organic matter was registered with respect to the fermenters working under acidic conditions. This fact was not reflected in a higher VFA production when using OFMSW as feedstock, probably due to free ammonia inhibition, since OFMSW was mixed in the MBT plant with supernatant from anaerobic digestion of this biowaste. However, when using FW, alkaline conditions lead to an enhanced VFA production with respect to the reactor working under acidic conditions, being acetic acid the predominant product, which represented up to 91% of the VFA spectrum obtained.
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Project description:Like many populated urban agglomerations in Africa, Kinshasa, the capital of Democratic Republic of Congo, faces several challenges to manage its exponentially growing Municipal Solid Waste. With its 12, 000, 000 people daily generating 7800 tons of Municipal Solid waste, the city still struggles with basic services such as waste collection and sanitary landfill. This causes major social, environmental and health related issues. With the aim of contributing to the implementation of a better management system in Kinshasa, this study evaluates the environmental impact and the cost of the existing waste management framework and proposes 6 alternative scenarios. Each scenario attempts to optimize Greenhouse gas emissions and cost, using the Life Cycle Assessment approach. Results show that the current municipal solid waste management in Kinshasa city emits 640,673 tons of CO2 equivalent per year and costs a total of 17, 776, 169.78USD yearly. Focusing on increasing waste collection coverage and recycling activities in the proposed 6 scenarios, scenario 4 where all municipal solid waste is collected, produces 4,042,402 tons of CO2 equivalent per year and costs 143, 296, 983.4 yearly. In scenario 7, considered the most optimized management model for Kinshasa in this study, municipal solid waste is valorized through different treatment processes and atmospheric pollution reaches 2,835,491 tons of CO2 equivalent yearly, with a management cost of 152, 790, 779.4 USD/year. This study finds that the optimization of the Municipal Solid Waste management system in Kinshasa city causes the atmospheric pollution in terms of CO2 equivalent to decrease by half, when all waste is collected. Landfill diversion rate reaches up to 70%, but the overall MSW management cost increases by almost eight times as much as the current operational cost. The optimization of the management system is done by increasing waste collection coverage and implementing diverse streams of waste valorization. Despite the wide use of the Life Cycle Assessment method in waste management and decision making, this method has not yet been, to the best knowledge of the authors, applied in estimating Greenhouse gas emissions and cost of the Municipal Solid Waste in the specific context of Kinshasa city.
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Project description:This article contains (i) a set of spreadsheets with data compiled from municipal sanitation or solid waste plans, and (ii) data of the individual and aggregate performance indicators. These indicators have been published in the Journal of Cleaner Production in the article entitled "A municipal solid waste indicator for environmental impact: assessment and identification of best management practices." The data contained in the spreadsheets are divided as follows: worksheet 1 includes the municipal solid waste generation data from the Brazilian municipalities studied; worksheet 2 presents the individual indicators that form the aggregate indicator; worksheet 3 presents the aggregate indicator and the classification of the municipalities; worksheet 4 provides data correlation; worksheets 5 to 10 depict boxplot graphs of the data; and worksheets 11 to 14 present graphs of individual indicators on a per capita basis and the ranking of municipalities.
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Project description:This paper presents the analysis of a pilot anaerobic digestion plant that operates with organic fraction of municipal solid waste (OFMSW) from a wholesale market and can treat up to 500 kg d-1. The process was monitored for a period of 524 days during which the residue was characterized and the biogas production and methane content were recorded. The organic load rate (OLR) of volatile solids (VS) was 0.89 kg m-3 d-1 and the Hydraulic Retention Time (HRT) was 25 d during the process. The yield was 82 Nm3 tons OFMSW-1 biogas, equivalent to 586 Nm3 tons CH4 VS-1. The results obtained in the pilot plant were used to carry out a technical-economic evaluation of a plant that treats 50 tons of OFMSW from wholesale markets. A production of 3769 Nm3 d-1 of biogas and 2080 Nm3 d-1 of methane is estimated, generating 35.1 MWh d-1 when converted to electricity.
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