RAD51 separation of function mutation disables replication fork maintenance but preserves DSB repair.
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ABSTRACT:
SUBMITTER: Son MY
PROVIDER: S-EPMC10993188 | biostudies-literature | 2024 Apr
REPOSITORIES: biostudies-literature
Publications
RAD51 separation of function mutation disables replication fork maintenance but preserves DSB repair.
Son Mi Young MY Belan Ondrej O Spirek Mario M Cibulka Jakub J Nikulenkov Fedor F Kim You Young YY Hwang Sunyoung S Myung Kyungjae K Montagna Cristina C Kim Tae Moon TM Krejci Lumir L Hasty Paul P
iScience 20240316 4
Homologous recombination (HR) protects replication forks (RFs) and repairs DNA double-strand breaks (DSBs). Within HR, BRCA2 regulates RAD51 via two interaction regions: the BRC repeats to form filaments on single-stranded DNA and exon 27 (Ex27) to stabilize the filament. Here, we identified a RAD51 S181P mutant that selectively disrupted the RAD51-Ex27 association while maintaining interaction with BRC repeat and proficiently forming filaments capable of DNA binding and strand invasion. Interes ...[more]
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