Substrate specificity of a ketosynthase domain involved in bacillaene biosynthesis.
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ABSTRACT:
SUBMITTER: Yin Z
PROVIDER: S-EPMC10999986 | biostudies-literature | 2024
REPOSITORIES: biostudies-literature
Publications
Substrate specificity of a ketosynthase domain involved in bacillaene biosynthesis.
Yin Zhiyong Z Dickschat Jeroen S JS
Beilstein journal of organic chemistry 20240405
An isotopic labelling method was developed to investigate substrate binding by ketosynthases, exemplified by the second ketosynthase of the polyketide synthase BaeJ involved in bacillaene biosynthesis (BaeJ-KS2). For this purpose, both enantiomers of a <sup>13</sup>C-labelled <i>N</i>-acetylcysteamine thioester (SNAC ester) surrogate of the proposed natural intermediate of BaeJ-KS2 were synthesised, including an enzymatic step with glutamate decarboxylase, and incubated with BaeJ-KS2. Substrate ...[more]
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