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Effects of cytochalasin D on relaxation process of skinned taenia cecum and carotid artery from guinea pig.


ABSTRACT: Actin linked regulatory mechanisms are known to contribute contraction/relaxation in smooth muscle. In order to clarify whether modulation of polymerization/depolymerization of actin filaments affects relaxation process, we examined the effects of cytochalasin D on relaxation process by Ca2+ removal after Ca2+-induced contraction of β-escin skinned (cell membrane permeabilized) taenia cecum and carotid artery preparations from guinea pigs. Cytochalasin D, an inhibitor of actin polymerization, significantly suppressed the force during relaxation both in skinned taenia cecum and carotid artery. The data fitting analysis of the relaxation processes indicates that cytochalasin D accelerates slow (latch-like) bridge dissociation. Cytochalasin D seems to directly disrupts actin filament organization or its length, resulting in modulation of actin filament structure that prevents myosin binding.

SUBMITTER: Mihashi S 

PROVIDER: S-EPMC11007923 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature

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Effects of cytochalasin D on relaxation process of skinned taenia cecum and carotid artery from guinea pig.

Mihashi Satoko S   Watanabe Masaru M  

The journal of physiological sciences : JPS 20240410 1


Actin linked regulatory mechanisms are known to contribute contraction/relaxation in smooth muscle. In order to clarify whether modulation of polymerization/depolymerization of actin filaments affects relaxation process, we examined the effects of cytochalasin D on relaxation process by Ca<sup>2+</sup> removal after Ca<sup>2+</sup>-induced contraction of β-escin skinned (cell membrane permeabilized) taenia cecum and carotid artery preparations from guinea pigs. Cytochalasin D, an inhibitor of ac  ...[more]

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