Protocol to longitudinally quantify SARS-CoV-2 RNA in wastewater using RT-qPCR and pepper mild mottle virus normalization.
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ABSTRACT:
SUBMITTER: Jimenez BS
PROVIDER: S-EPMC11011218 | biostudies-literature | 2024 Apr
REPOSITORIES: biostudies-literature
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Protocol to longitudinally quantify SARS-CoV-2 RNA in wastewater using RT-qPCR and pepper mild mottle virus normalization.
Jimenez Bryan Sanchez BS Sterling Trinity T Brown Austin A Modica Brian B Gibson Kaylee K Collins Hannah H Koch Carolyn C Schwarz Tyler T Dye Kristine N KN
STAR protocols 20240409 2
Wastewater surveillance allows severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection levels to be tracked in a community. Here, we present a protocol to longitudinally quantify SARS-CoV-2 RNA in wastewater using quantitative reverse-transcription PCR (RT-qPCR) and pepper mild mottle virus (PMMoV) normalization. We describe steps for the pasteurization of wastewater samples, solids separation, supernatant filtration, viral precipitation and concentration, and RNA extraction. We t ...[more]
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