Ontology highlight
ABSTRACT:
SUBMITTER: Chen Y
PROVIDER: S-EPMC11269290 | biostudies-literature | 2024 Jul
REPOSITORIES: biostudies-literature
Chen Yibao Y Yan Bingjie B Chen Weizhong W Zhang Xue X Liu Zhengjie Z Zhang Qing Q Li Lulu L Hu Ming M Zhao Xiaonan X Xu Xiaohui X Lv Qianghua Q Luo Yanbo Y Cai Yumei Y Liu Yuqing Y
iScience 20240606 7
<i>Pseudomonas aeruginosa</i> is a common opportunistic pathogen. The potential efficacy of phage therapy has attracted the attention of researchers, but efficient gene-editing tools are lacking, limiting the study of their biological properties. Here, we designed a type V CRISPR-Cas12a system for the gene editing of <i>P. aeruginosa</i> phages. We first evaluated the active cutting function of the CRISPR-Cas12a system <i>in vitro</i> and discovered that it had a higher gene-cutting efficiency t ...[more]