Dataset Information

Gut Microbiota and Cytokine Profile in Cirrhosis.

ABSTRACT:

Background and aims

Gut dysbiosis and abnormal cytokine profiles are common in cirrhosis. This study aimed to evaluate the correlations between them.

Methods

In the blood plasma of cirrhosis patients and controls, 27 cytokines were examined using a multiplex assay. The plasma levels of nitrites (stable metabolites of the endothelial dysfunction biomarker nitric oxide) and lipopolysaccharide (LPS) were examined. The fecal microbiota was assessed by 16S rRNA gene sequencing.

Results

Levels of IL-1b, IL-2, IL-6, IL-13, IP-10, IFN-g, TNF-a, LPS, and nitrites were higher in cirrhosis patients than in controls, while levels of IL-4, IL-7, and PDGF-BB were lower. The LPS level was directly correlated with the levels of IL-1b, IL1-Ra, IL-9, IL-17, PDGF-BB, IL-6, TNF-a, and nitrites. The nitrite level was significantly directly correlated with the levels of TNF-a, GM-CSF, IL-17, and IL-12, and inversely correlated with the IL-7 level. TNF-a levels were directly correlated with ascites severity and the abundance of Negativicutes, Enterobacteriaceae, Veillonellaceae, and Klebsiella, while inversely correlated with the abundance of Firmicutes, Clostridia, and Subdoligranulum. IFN-g levels were directly correlated with the abundance of Bacteroidaceae, Lactobacillaceae, Bacteroides, and Megasphaera, and inversely correlated with the abundance of Verrucomicrobiota, Akkermansiaceae, Coriobacteriaceae, Akkermansia, Collinsella, and Gemella. IL-1b levels were directly correlated with the abundance of Comamonadaceae and Enterobacteriaceae and inversely correlated with the abundance of Marinifilaceae and Dialister. IL-6 levels were directly correlated with the abundance of Enterobacteriaceae, hepatic encephalopathy, and ascites severity, and inversely correlated with the abundance of Peptostreptococcaceae, Streptococcaceae, and Streptococcus.

Conclusions

The abundance of harmful gut microbiota taxa and endotoxinemia directly correlates with the levels of proinflammatory cytokines.

SUBMITTER: Efremova I

PROVIDER: S-EPMC11310756 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Publications

Gut Microbiota and Cytokine Profile in Cirrhosis.

Efremova Irina I Maslennikov Roman R Kudryavtseva Anna A Avdeeva Anastasia A Krasnov George G Diatroptov Mikhail M Bakhitov Vyacheslav V Aliev Salekh S Sedova Natalia N Fedorova Maria M Poluektova Elena E Zolnikova Oxana O Aliev Nariman N Levshina Anna A Ivashkin Vladimir V

Journal of clinical and translational hepatology 20240628 8

<h4>Background and aims</h4>Gut dysbiosis and abnormal cytokine profiles are common in cirrhosis. This study aimed to evaluate the correlations between them.<h4>Methods</h4>In the blood plasma of cirrhosis patients and controls, 27 cytokines were examined using a multiplex assay. The plasma levels of nitrites (stable metabolites of the endothelial dysfunction biomarker nitric oxide) and lipopolysaccharide (LPS) were examined. The fecal microbiota was assessed by 16S rRNA gene sequencing.<h4>Resu ...[more]

PMID: 39130620

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Dataset Information

Gut Microbiota and Cytokine Profile in Cirrhosis.

Background and aims

Methods

Results

Conclusions

Publications

Gut Microbiota and Cytokine Profile in Cirrhosis.

Similar Datasets

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