Unknown

Dataset Information

0

Opsonization-independent antigen-specific recognition by myeloid phagocytes expressing monoclonal antibodies.


ABSTRACT: This report demonstrates a novel class of innate immune cells designated "variable immunoreceptor-expressing myeloids" (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid cells unrelated to lymphocytes. We visualize the phenotype of B-VIREMs that are capable of genetically recombining and expressing antibody genes, the exclusive hallmark function of B lymphocytes. These cells, designated B-VIREMs, display monoclonal antibody cell surface signatures and regularly circulate in the blood of healthy individuals. Single-cell data reveal clonal expansion of circulating B-VIREMs as a dynamic response to disease stimuli. Live-cell imaging models suggest that B-VIREMs load their own Fc receptors with endogenous antibodies during vesicle transport to the cell surface. A first cloned B-VIREM-derived antibody (Vab1) specifically binds stomatin, a ubiquitous scaffold protein that is strictly expressed intracellularly, allowing Vab1-bearing macrophages to phagocytose cell debris without requiring prior opsonization. Our results suggest important antigen-specific tissue maintenance functionalities in these innate immune cells.

SUBMITTER: Neumaier M 

PROVIDER: S-EPMC11314243 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications


This report demonstrates a novel class of innate immune cells designated "variable immunoreceptor-expressing myeloids" (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid cells unrelated to lymphocytes. We visualize the phenotype of B-VIREMs that are capable of genetically recombining and expressing antibody genes, the exclusive hallmark function of B lymphocytes. These cells, designated B-VIREMs, display monoclonal antibody cell  ...[more]

Similar Datasets

| S-EPMC3078118 | biostudies-literature
| S-EPMC7021589 | biostudies-literature
2019-03-02 | GSE127729 | GEO
| S-EPMC7948472 | biostudies-literature
| S-EPMC3520816 | biostudies-literature
| S-EPMC2750034 | biostudies-literature
| S-EPMC6798180 | biostudies-literature
| S-EPMC5551578 | biostudies-literature
| S-EPMC4911382 | biostudies-literature
| S-EPMC2949385 | biostudies-literature