Dataset Information

Real-time intelligent classification of COVID-19 and thrombosis via massive image-based analysis of platelet aggregates.

ABSTRACT: Microvascular thrombosis is a typical symptom of COVID-19 and shows similarities to thrombosis. Using a microfluidic imaging flow cytometer, we measured the blood of 181 COVID-19 samples and 101 non-COVID-19 thrombosis samples, resulting in a total of 6.3 million bright-field images. We trained a convolutional neural network to distinguish single platelets, platelet aggregates, and white blood cells and performed classical image analysis for each subpopulation individually. Based on derived single-cell features for each population, we trained machine learning models for classification between COVID-19 and non-COVID-19 thrombosis, resulting in a patient testing accuracy of 75%. This result indicates that platelet formation differs between COVID-19 and non-COVID-19 thrombosis. All analysis steps were optimized for efficiency and implemented in an easy-to-use plugin for the image viewer napari, allowing the entire analysis to be performed within seconds on mid-range computers, which could be used for real-time diagnosis.

SUBMITTER: Zhang C

PROVIDER: S-EPMC11331588 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Real-time intelligent classification of COVID-19 and thrombosis via massive image-based analysis of platelet aggregates.

Zhang Chenqi C Herbig Maik M Zhou Yuqi Y Nishikawa Masako M Shifat-E-Rabbi Mohammad M Kanno Hiroshi H Yang Ruoxi R Ibayashi Yuma Y Xiao Ting-Hui TH Rohde Gustavo K GK Sato Masataka M Kodera Satoshi S Daimon Masao M Yatomi Yutaka Y Goda Keisuke K

Cytometry. Part A : the journal of the International Society for Analytical Cytology 20230221 6

Microvascular thrombosis is a typical symptom of COVID-19 and shows similarities to thrombosis. Using a microfluidic imaging flow cytometer, we measured the blood of 181 COVID-19 samples and 101 non-COVID-19 thrombosis samples, resulting in a total of 6.3 million bright-field images. We trained a convolutional neural network to distinguish single platelets, platelet aggregates, and white blood cells and performed classical image analysis for each subpopulation individually. Based on derived sing ...[more]

PMID: 36772915

Similar Datasets

Project description:Rationale: High-intensity focused ultrasound (HIFU) therapy represents a noninvasive surgical approach to treat uterine fibroids. The operation of HIFU therapy relies on the information provided by medical images. In current HIFU therapy, all operations such as positioning of the lesion in magnetic resonance (MR) and ultrasound (US) images are manually performed by specifically trained doctors. Manual processing is an important limitation of the efficiency of HIFU therapy. In this paper, we aim to provide an automatic and accurate image guidance system, intelligent diagnosis, and treatment strategy for HIFU therapy by combining multimodality information. Methods: In intelligent HIFU therapy, medical information and treatment strategy are automatically processed and generated by a real-time image guidance system. The system comprises a novel multistage deep convolutional neural network for preoperative diagnosis and a nonrigid US lesion tracking procedure for HIFU intraoperative image-assisted treatment. In the process of intelligent therapy, the treatment area is determined from the autogenerated lesion area. Based on the autodetected treatment area, the HIFU foci are distributed automatically according to the treatment strategy. Moreover, an image-based unexpected movement warning and other physiological monitoring are used during the intelligent treatment procedure for safety assurance. Results: In the experiment, we integrated the intelligent treatment system on a commercial HIFU treatment device, and eight clinical experiments were performed. In the clinical validation, eight randomly selected clinical cases were used to verify the feasibility of the system. The results of the quantitative experiment indicated that our intelligent system met the HIFU clinical tracking accuracy and speed requirements. Moreover, the results of simulated repeated experiments confirmed that the autodistributed HIFU focus reached the level of intermediate clinical doctors. Operations performed by junior- or middle-level operators with the assistance of the proposed system can reach the level of operation performed by senior doctors. Various experiments prove that our proposed intelligent HIFU therapy process is feasible for treating common uterine fibroid cases. Conclusion: We propose an intelligent HIFU therapy for uterine fibroid which integrates multiple medical information processing procedures. The experiment results demonstrated that the proposed procedures and methods can achieve monitored and automatic HIFU diagnosis and treatment. This research provides a possibility for intelligent and automatic noninvasive therapy for uterine fibroid.

Project description:Waste management handles all kinds of waste, including household, industrial, municipal, organic, biomedical, biological, and radioactive wastes. People still face challenges in proper disposal methods for different types of waste, including landfill-bound items, recyclable materials, and biodegradable waste. Inadequate waste management poses a significant and multifaceted global challenge. The conventional method of segregating waste is a time-consuming and ineffective method that wastes human power and money. To address this issue in real time, sophisticated and sustainable waste management systems need to be implemented. The latest advancements in computer vision and deep learning offer efficient solutions for effective recycling and waste management. Existing deep learning models exhibited various limitations, such as detection accuracy and computational inefficiency, particularly when dealing with objects of varying sizes and exhibiting high degrees of visual similarity. These limitations generate various challenges in effectively capturing and representing the nuanced features of visually similar objects. To address this problem, we proposed the stacking of an enhanced Swin Transformer, improved ConvNeXt, and a spatial attention mechanism. The enhanced Swin transformers incorporate two key components- hierarchical feature extraction and shifting window mechanism to extract the global features from the garbage images effectively. The shifting window mechanism extracts the most important features from various regions of the images to identify the objects. In contrast, the hierarchical feature extraction captures long-range dependencies within the image to effectively identify different types of garbage. The improved ConvNext block with optimized parameterization extracts the local features of the image. This enhanced feature extraction capability enables the model to effectively discern fine-grained details of individual garbage particles, such as shape, texture, and subtle variations in color and appearance, leading to more accurate classification results. When we evaluated the performance of the proposed model using the publicly available Garbage Classification dataset, it attained 98.97% accuracy, 98.42% Precision, and 98.61% Recall. Due to its lightweight and low computational time and power, the proposed model surpasses the existing state-of-the-art deep learning models.

Dataset Information

Real-time intelligent classification of COVID-19 and thrombosis via massive image-based analysis of platelet aggregates.

Publications

Real-time intelligent classification of COVID-19 and thrombosis via massive image-based analysis of platelet aggregates.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets