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Exploring the genetic diversity and population structure of aerial yams (Dioscorea bulbifera L.) DArT-seq and agronomic traits.

ABSTRACT: Dioscorea bulbifera is an edible yam specie with aerial bulbils. Assessing the genetic diversity of D. bulbifera accession for cultivation and breeding purposes is essential for it genetic improvement, especially where the crop faces minimal attention. The aims of this study was to assess the genetic diversity of Dioscorea bulbifera accessions collected from Nigeria and accessions maintained at the genebank of International Institute of Tropical Agriculture (IITA) Ibadan. Accessions were profiled using quatitative and qualitative phenotypic traits and Diversity Array Technology SNP-markers. Multivariate analysis based phenotypic traits revealed high variability among the evaluated accessions and all phenotypic traits assessed were useful in discriminating the aerial yam accessions. Clustering analysis based phenotypic traits revealed the presence of two well defined clusters. Using DArT-Seq marker, the 94 accessions were classified into three genetic group through the admixture and the phylogeny analysis. The comparision of phenotypic and genotypic clustering revealed inconsistency membership across the two clustering methods. The study established a baseline for the selection of parental lines from the genetic groups for genetic improvement of the D. bulbifera.

SUBMITTER: Ekaette E

PROVIDER: S-EPMC11343425 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature

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Exploring the genetic diversity and population structure of aerial yams (Dioscorea bulbifera L.) DArT-seq and agronomic traits.

Ekaette Eunice E Nwofia Emeka E Okocha Peter P Nnnabue Ikenna I Eluwa Kenneth K Obidiegwu Jude J Agre Paterne A PA

PloS one 20240823 8

Dioscorea bulbifera is an edible yam specie with aerial bulbils. Assessing the genetic diversity of D. bulbifera accession for cultivation and breeding purposes is essential for it genetic improvement, especially where the crop faces minimal attention. The aims of this study was to assess the genetic diversity of Dioscorea bulbifera accessions collected from Nigeria and accessions maintained at the genebank of International Institute of Tropical Agriculture (IITA) Ibadan. Accessions were profile ...[more]

PMID: 39178185

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Project description:BackgroundDioscorea bulbifera is an African medicinal plant used to treat microbial infections. In the present study, the methanol extract, fractions (DBB1 and DBB2) and six compounds isolated from the bulbils of D. bulbifera, namely bafoudiosbulbins A (1), B (2), C (3), F (4), G (5) and 2,7-dihydroxy-4-methoxyphenanthrene (6), were tested for their antimicrobial activities against Mycobacteria and Gram-negative bacteria involving multidrug resistant (MDR) phenotypes expressing active efflux pumps.MethodsThe microplate alamar blue assay (MABA) and the broth microdilution methods were used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the above samples.ResultsThe results of the MIC determinations indicated that when tested alone, the crude extract, fractions DBB1 and DBB2 as well as compounds 2 to 5 were able to prevent the growth of all the fifteen studied microorganisms, within the concentration range of 8 to 256 μg/mL. The lowest MIC value for the methanol extract and fractions (16 μg/mL) was obtained with DBB1 and DBB2 on E, coli AG100A and DBB2 on Mycobacterium tuberculosis MTCS2. The lowest value for individual compounds (8 μg/mL) was recorded with compound 3 on M. smegmatis and M. tuberculosis ATCC and MTCS2 strains respectively. The activity of the samples on many MDR bacteria such as Enterobacter aerogenes EA289, CM64, Klebsiella pneumoniae KP63 and Pseudomonas aeruginosa PA124 was better than that of chloramphenicol. When tested in the presence of the efflux pump inhibitor against MDR Gram-negative bacteria, the activity of most of the samples increased. MBC values not greater than 512 μg/mL were recorded on all studied microorganisms with fraction DBB2 and compounds 2 to 5.ConclusionsThe overall results of the present investigation provided evidence that the crude extract D. bulbifera as well as some of the compounds and mostly compounds 3 could be considered as potential antimicrobial drugs to fight against MDR bacteria.

Dataset Information

Exploring the genetic diversity and population structure of aerial yams (Dioscorea bulbifera L.) DArT-seq and agronomic traits.

Publications

Exploring the genetic diversity and population structure of aerial yams (Dioscorea bulbifera L.) DArT-seq and agronomic traits.

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