Ontology highlight
ABSTRACT:
SUBMITTER: Mendes MV
PROVIDER: S-EPMC1134766 | biostudies-literature | 2005 Feb
REPOSITORIES: biostudies-literature
Mendes Marta V MV Antón Nuria N Martín Juan F JF Aparicio Jesús F JF
The Biochemical journal 20050201 Pt 1
The biosynthesis of the antifungal agent pimaricin by Streptomyces natalensis has been proposed to involve a cytochrome P450 encoded by the gene pimD. Pimaricin is derived from its immediate precursor de-epoxypimaricin by epoxidation of the C-4-C-5 double bond on the macrolactone ring. We have overproduced PimD with a N-terminal His6 affinity tag in Escherichia coli and purified the enzyme for kinetic analysis. The protein showed a reduced CO-difference spectrum with a Soret maximum at 450 nm, i ...[more]