Homodimerization of calpain 3 penta-EF-hand domain.
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ABSTRACT:
SUBMITTER: Ravulapalli R
PROVIDER: S-EPMC1138966 | biostudies-literature | 2005 Jun
REPOSITORIES: biostudies-literature
Publications
Homodimerization of calpain 3 penta-EF-hand domain.
Ravulapalli Ravikiran R Diaz Beatriz Garcia BG Campbell Robert L RL Davies Peter L PL
The Biochemical journal 20050601 Pt 2
Calpains 1 and 2 are heterodimeric proteases in which large (relative molecular mass M(r) 80000) and small (M(r) 28000) subunits are linked through their respective PEF (penta-EF-hand) domains. The skeletal muscle-specific calpain 3 is believed not to form a heterodimer with the small subunit but might homodimerize through its PEF domain. Size-exclusion chromatography and analytical ultracentrifugation of the recombinant PEF domain of calpain 3 show that it forms a stable homodimer that does not ...[more]
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