Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages.

ABSTRACT: Macrophages are central to host defense against microbes, but intracellular pathogens have evolved to evade their antimicrobial functions. Mycobacterium tuberculosis (MTB) has successfully exploited macrophages as its primary niche in vivo, but the bacterial genome-wide requirements that promote its intracellular survival remain undefined. Here we comprehensively identify the MTB genes required for survival by screening for transposon mutants that fail to grow within primary macrophages. We identify mutants showing decreased growth in macrophage environments that model stages of the host immune response. By systematically analyzing several biologically relevant data sets, we have been able to identify putative pathways that could not be predicted by genome organization alone. In one exampl

SUBMITTER: Rengarajan J 

PROVIDER: S-EPMC1142121 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature