Rescue of recombinant Thogoto virus from cloned cDNA.
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ABSTRACT:
SUBMITTER: Wagner E
PROVIDER: S-EPMC114495 | biostudies-literature | 2001 Oct
REPOSITORIES: biostudies-literature
Publications
Rescue of recombinant Thogoto virus from cloned cDNA.
Wagner E E Engelhardt O G OG Gruber S S Haller O O Kochs G G
Journal of virology 20011001 19
Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome consisting of six negative-stranded RNA segments. To rescue a recombinant THOV, the viral structural proteins were produced from expression plasmids by means of a vaccinia virus expressing the T7 RNA polymerase. Genomic virus RNAs (vRNAs) were generated from plasmids under the control of the RNA polymerase I promoter. Using this system, we could efficiently recover recombinant THOV following transfection of 12 plasmids into ...[more]
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