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Vaccination with attenuated simian immunodeficiency virus by DNA inoculation.

ABSTRACT: Delivering attenuated lentivirus vaccines as proviral DNA would be simple and inexpensive. Inoculation of macaques with wild-type simian immunodeficiency virus strain mac239 (SIV(mac239)) DNA or SIV(mac239) DNA containing a single deletion in the 3' nef-long terminal repeat overlap region (nef/LTR) led to sustained SIV infections and AIDS. Injection of SIV(mac239) DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuated SIV infections and substantial protection against subsequent mucosal SIV(mac251) challenge.

SUBMITTER: Kent SJ

PROVIDER: S-EPMC114784 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature

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Vaccination with attenuated simian immunodeficiency virus by DNA inoculation.

Kent S J SJ Dale C J CJ Preiss S S Mills J J Campagna D D Purcell D F DF

Journal of virology 20011201 23

Delivering attenuated lentivirus vaccines as proviral DNA would be simple and inexpensive. Inoculation of macaques with wild-type simian immunodeficiency virus strain mac239 (SIV(mac239)) DNA or SIV(mac239) DNA containing a single deletion in the 3' nef-long terminal repeat overlap region (nef/LTR) led to sustained SIV infections and AIDS. Injection of SIV(mac239) DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuated SIV infections and substantial protection ...[more]

PMID: 11689679

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