Surface display of recombinant proteins on Bacillus thuringiensis spores.
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ABSTRACT:
SUBMITTER: Du C
PROVIDER: S-EPMC1151829 | biostudies-literature | 2005 Jun
REPOSITORIES: biostudies-literature
Publications
Surface display of recombinant proteins on Bacillus thuringiensis spores.
Du Cheng C Chan Wing C WC McKeithan Timothy W TW Nickerson Kenneth W KW
Applied and environmental microbiology 20050601 6
The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulation-specific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one ...[more]
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