Ontology highlight
ABSTRACT:
SUBMITTER: Deng YM
PROVIDER: S-EPMC115187 | biostudies-literature | 2000 Dec
REPOSITORIES: biostudies-literature
Deng Y M YM Lee J H JH Moran C C Jin J H JH Tuch B E BE Rawlinson W D WD
Nucleic acids research 20001201 23
A simple and effective method based upon semi-specific PCR followed by cloning has been developed. Chromosomal mapping of the generated fragment on a somatic cell hybrid panel identifies the chromosomal position, and yields a unique sequence tag for the site. Using this method, the chromosomal location of one porcine endogenous retrovirus (PERV) was determined. The porcine genomic sequences were first amplified by PCR using a PERV-specific primer and a porcine short interspersed nuclear element ...[more]