Ontology highlight
ABSTRACT:
SUBMITTER: Crut A
PROVIDER: S-EPMC1153714 | biostudies-literature | 2005 Jun
REPOSITORIES: biostudies-literature
Crut Aurélien A Géron-Landre Bénédicte B Bonnet Isabelle I Bonneau Stéphane S Desbiolles Pierre P Escudé Christophe C
Nucleic acids research 20050620 11
Observation of DNA-protein interactions by single molecule fluorescence microscopy is usually performed by using fluorescent DNA binding agents. However, such dyes have been shown to induce cleavage of the DNA molecule and perturb its interactions with proteins. A new method for the detection of surface-attached DNA molecules by fluorescence microscopy is introduced in this paper. Biotin- and/or digoxigenin-modified DNA fragments are covalently linked at both extremities of a DNA molecule via se ...[more]