Derivation of multipotent mesenchymal precursors from human embryonic stem cells.
Ontology highlight
ABSTRACT: Background
Human embryonic stem cells provide access to the earliest stages of human development and may serve as a source of specialized cells for regenerative medicine. Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic stem cells into tissue-restricted precursors.Methods and findings
Here, we present culture conditions for the derivation of unlimited numbers of pure mesenchymal precursors from human embryonic stem cells and demonstrate multilineage differentiation into fat, cartilage, bone, and skeletal muscle cells.Conclusion
Our findings will help to elucidate the mechanism of mesoderm specification during embryonic stem cell differentiation and provide a platform to efficiently generate specialized human mesenchymal cell types for future clinical applications.
SUBMITTER: Barberi T
PROVIDER: S-EPMC1160574 | biostudies-literature | 2005 Jun
REPOSITORIES: biostudies-literature
Publications
Derivation of multipotent mesenchymal precursors from human embryonic stem cells.
Barberi Tiziano T Willis Lucy M LM Socci Nicholas D ND Studer Lorenz L
PLoS medicine 20050628 6
<h4>Background</h4>Human embryonic stem cells provide access to the earliest stages of human development and may serve as a source of specialized cells for regenerative medicine. Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic stem cells into tissue-restricted precursors.<h4>Methods and findings</h4>Here, we present culture conditions for the derivation of unlimited numbers of pure mesenchymal precursors from human embryonic stem cells and demonstrate ...[more]
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