Ontology highlight
ABSTRACT:
SUBMITTER: Wang YM
PROVIDER: S-EPMC1168954 | biostudies-literature | 2005 Jul
REPOSITORIES: biostudies-literature
Wang Y M YM Tegenfeldt Jonas O JO Reisner W W Riehn R R Guan Xiao-Juan XJ Guo Ling L Golding Ido I Cox Edward C EC Sturm James J Austin Robert H RH
Proceedings of the National Academy of Sciences of the United States of America 20050630 28
We have performed single-molecule studies of GFP-LacI repressor proteins bound to bacteriophage lambda DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to be 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the repress ...[more]