Ontology highlight
ABSTRACT:
SUBMITTER: Zorin B
PROVIDER: S-EPMC1168964 | biostudies-literature | 2005 Jul
REPOSITORIES: biostudies-literature
Zorin Boris B Hegemann Peter P Sizova Irina I
Eukaryotic cell 20050701 7
Homologous DNA recombination (HR) allows the deletion (knockout), repair (rescuing), and modification of a selected gene, thereby rendering a functional analysis of the gene product possible. However, targeting of nuclear genes has been an inefficient process in most eukaryotes, including algae, plants, and animals, due to the dominance of integration of the applied DNA into nonhomologous regions of the genome. We have shown for the green alga Chlamydomonas reinhardtii by repairing a previously ...[more]