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ABSTRACT:
SUBMITTER: Fiedler E
PROVIDER: S-EPMC117350 | biostudies-literature | 2002 Jan
REPOSITORIES: biostudies-literature
Fiedler Erik E Thorell Stina S Sandalova Tatyana T Golbik Ralph R König Stephan S Schneider Gunter G
Proceedings of the National Academy of Sciences of the United States of America 20020102 2
Kinetic and spectroscopic data indicated that addition of the donor substrate hydroxypyruvate to the thiamin diphosphate (ThDP)-dependent enzyme transketolase (TK) led to the accumulation of the alpha-carbanion/enamine of (alpha,beta-dihydroxyethyl) ThDP, the key reaction intermediate in enzymatic thiamin catalysis. The three-dimensional structure of this intermediate trapped in the active site of yeast TK was determined to 1.9-A resolution by using cryocrystallography. The electron density sugg ...[more]