Ontology highlight
ABSTRACT:
SUBMITTER: Pastor MT
PROVIDER: S-EPMC117354 | biostudies-literature | 2002 Jan
REPOSITORIES: biostudies-literature
Pastor Maria Teresa MT López de la Paz Manuela M Lacroix Emmanuel E Serrano Luis L Pérez-Payá Enrique E
Proceedings of the National Academy of Sciences of the United States of America 20020108 2
Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-a ...[more]