Unknown

Dataset Information

0

Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice.


ABSTRACT: Over the past decade xenotransplantation systems have been used with increasing success to gain a better understanding of human cells that are able to initiate and maintain the hematopoietic system in vivo. The nonobese diabetic/severe combined immunodeficiency (SCID) mouse has been a particularly useful model. Human cells capable of hematopoietic repopulation in this mouse, termed SCID-repopulating cells, have been assumed to represent the most primitive elements of the hematopoietic system, responsible for long-term maintenance of hematopoiesis. However, we demonstrate that SCID-repopulating cells present in the CD34(+) cell fraction of cord blood can be segregated into subpopulations with distinct repopulation characteristics. CD34(+)/CD38(+) progenitors can repopulate recipients rapidly, but can only maintain the graft for 12 weeks or less and have no secondary repopulation potential. Conversely, the more primitive CD34(+)/CD38(-) subpopulation repopulates recipients more gradually, can maintain the graft for at least 20 weeks, and contains cells with serial repopulation potential throughout the engraftment period. Additionally, a much higher frequency of T cell precursors are found among SCID-repopulating cells in the CD34(+)/CD38(-) subpopulation. These findings demonstrate that cells with variable repopulation potential comprise the human CD34(+) population and that short- and long-term potential of human precursors can be evaluated in the mouse model.

SUBMITTER: Hogan CJ 

PROVIDER: S-EPMC117574 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice.

Hogan Christopher J CJ   Shpall Elizabeth J EJ   Keller Gordon G  

Proceedings of the National Academy of Sciences of the United States of America 20020101 1


Over the past decade xenotransplantation systems have been used with increasing success to gain a better understanding of human cells that are able to initiate and maintain the hematopoietic system in vivo. The nonobese diabetic/severe combined immunodeficiency (SCID) mouse has been a particularly useful model. Human cells capable of hematopoietic repopulation in this mouse, termed SCID-repopulating cells, have been assumed to represent the most primitive elements of the hematopoietic system, re  ...[more]

Similar Datasets

| S-EPMC4325197 | biostudies-literature
| S-EPMC4071379 | biostudies-literature
| S-EPMC2923186 | biostudies-literature
| S-EPMC4485368 | biostudies-literature
| S-EPMC2948507 | biostudies-literature
| S-EPMC7581443 | biostudies-literature
| S-EPMC3434672 | biostudies-literature
| S-EPMC4711970 | biostudies-literature
| S-EPMC5669860 | biostudies-literature
| S-EPMC3525660 | biostudies-literature