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Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

ABSTRACT: The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

SUBMITTER: Carter MG

PROVIDER: S-EPMC1175992 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature

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Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Carter Mark G MG Sharov Alexei A AA VanBuren Vincent V Dudekula Dawood B DB Carmack Condie E CE Nelson Charlie C Ko Minoru S H MS

Genome biology 20050630 7

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance. ...[more]

PMID: 15998450

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