Dataset Information

PSLIP: SVM based protein subcellular localization prediction using multiple physicochemical properties.

ABSTRACT:

Background

Protein subcellular localization is an important determinant of protein function and hence, reliable methods for prediction of localization are needed. A number of prediction algorithms have been developed based on amino acid compositions or on the N-terminal characteristics (signal peptides) of proteins. However, such approaches lead to a loss of contextual information. Moreover, where information about the physicochemical properties of amino acids has been used, the methods employed to exploit that information are less than optimal and could use the information more effectively.

Results

In this paper, we propose a new algorithm called pSLIP which uses Support Vector Machines (SVMs) in conjunction with multiple physicochemical properties of amino acids to predict protein subcellular localization in eukaryotes across six different locations, namely, chloroplast, cytoplasmic, extracellular, mitochondrial, nuclear and plasma membrane. The algorithm was applied to the dataset provided by Park and Kanehisa and we obtained prediction accuracies for the different classes ranging from 87.7%-97.0% with an overall accuracy of 93.1%.

Conclusion

This study presents a physicochemical property based protein localization prediction algorithm. Unlike other algorithms, contextual information is preserved by dividing the protein sequences into clusters. The prediction accuracy shows an improvement over other algorithms based on various types of amino acid composition (single, pair and gapped pair). We have also implemented a web server to predict protein localization across the six classes (available at http://pslip.bii.a-star.edu.sg/).

SUBMITTER: Sarda D

PROVIDER: S-EPMC1182350 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature

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Publications

pSLIP: SVM based protein subcellular localization prediction using multiple physicochemical properties.

Sarda Deepak D Chua Gek Huey GH Li Kuo-Bin KB Krishnan Arun A

BMC bioinformatics 20050617

<h4>Background</h4>Protein subcellular localization is an important determinant of protein function and hence, reliable methods for prediction of localization are needed. A number of prediction algorithms have been developed based on amino acid compositions or on the N-terminal characteristics (signal peptides) of proteins. However, such approaches lead to a loss of contextual information. Moreover, where information about the physicochemical properties of amino acids has been used, the methods ...[more]

PMID: 15963230

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