Ontology highlight
ABSTRACT:
SUBMITTER: Bromidge TJ
PROVIDER: S-EPMC1186973 | biostudies-literature | 2000 Aug
REPOSITORIES: biostudies-literature
Molecular pathology : MP 20000801 4
This report details a rapid method for screening the entire p53 coding region (exons 2-11). This method, based on the non-isotopic RNase cleavage assay, uses novel primer sequences and an adaptation of the MutationScreener method. A mutation in 20% of the sample was easily detectable by this method, whereas mutations below 50% were undetectable using the original method. Alterations to the wild-type p53 mRNA sequence were found in nine of the 130 patients with low grade lymphoproliferative disor ...[more]