Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.
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ABSTRACT: Background
Cryopreservation of PBMC and/or overnight shipping of samples are required for many clinical trials, despite their potentially adverse effects upon immune monitoring assays such as MHC-peptide tetramer staining, cytokine flow cytometry (CFC), and ELISPOT. In this study, we compared the performance of these assays on leukapheresed PBMC shipped overnight in medium versus cryopreserved PBMC from matched donors.Results
Using CMV pp65 peptide pool stimulation or pp65 HLA-A2 tetramer staining, there was significant correlation between shipped and cryopreserved samples for each assay (p
SUBMITTER: Maecker HT
PROVIDER: S-EPMC1190174 | biostudies-literature | 2005 Jul
REPOSITORIES: biostudies-literature
Publications
Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.
Maecker Holden T HT Moon James J Bhatia Sonny S Ghanekar Smita A SA Maino Vernon C VC Payne Janice K JK Kuus-Reichel Kristine K Chang Jennie C JC Summers Amanda A Clay Timothy M TM Morse Michael A MA Lyerly H Kim HK DeLaRosa Corazon C Ankerst Donna P DP Disis Mary L ML
BMC immunology 20050718
<h4>Background</h4>Cryopreservation of PBMC and/or overnight shipping of samples are required for many clinical trials, despite their potentially adverse effects upon immune monitoring assays such as MHC-peptide tetramer staining, cytokine flow cytometry (CFC), and ELISPOT. In this study, we compared the performance of these assays on leukapheresed PBMC shipped overnight in medium versus cryopreserved PBMC from matched donors.<h4>Results</h4>Using CMV pp65 peptide pool stimulation or pp65 HLA-A2 ...[more]
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