Ontology highlight
ABSTRACT:
SUBMITTER: Florio W
PROVIDER: S-EPMC120012 | biostudies-literature | 2002 Jul
REPOSITORIES: biostudies-literature
Florio W W Bottai D D Batoni G G Esin S S Pardini M M Maisetta G G Campa M M
Clinical and diagnostic laboratory immunology 20020701 4
Diagnosis of tuberculosis is time-consuming and requires infrastructures which are often not available in countries with high incidences of the disease. In the present study, an 82-kDa protein antigen was isolated by affinity chromatography and was identified by peptide mass fingerprinting as isocitrate dehydrogenase II, which is encoded by the icd2 gene of Mycobacterium bovis BCG. The icd2 gene of BCG was cloned by PCR, and the product of recombinant gene expression was purified and analyzed by ...[more]