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Protein "fingerprinting" in complex mixtures with peptoid microarrays.

ABSTRACT: We report here that microarrays comprised of several thousand peptoids (oligo-N-substituted glycines) are useful tools for the identification of proteins via a "fingerprinting" approach. By using maltose-binding protein, glutathione S-transferase, and ubiquitin, a specific and highly reproducible pattern of binding was observed when fluorescently labeled protein was hybridized to the array. A similar pattern was obtained when binding of an unlabeled protein to the array was visualized by secondary hybridization of a labeled antibody against that protein, showing that native proteins can be identified without the requirement for prior chemical labeling. This work suggests that small-molecule microarrays might be used for more complex fingerprinting assays of potential diagnostic value.

SUBMITTER: Reddy MM

PROVIDER: S-EPMC1200257 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature

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Protein "fingerprinting" in complex mixtures with peptoid microarrays.

Reddy M Muralidhar MM Kodadek Thomas T

Proceedings of the National Academy of Sciences of the United States of America 20050825 36

We report here that microarrays comprised of several thousand peptoids (oligo-N-substituted glycines) are useful tools for the identification of proteins via a "fingerprinting" approach. By using maltose-binding protein, glutathione S-transferase, and ubiquitin, a specific and highly reproducible pattern of binding was observed when fluorescently labeled protein was hybridized to the array. A similar pattern was obtained when binding of an unlabeled protein to the array was visualized by seconda ...[more]

PMID: 16123137

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