Biological function of the vaccinia virus Z-DNA-binding protein E3L: gene transactivation and antiapoptotic activity in HeLa cells.
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ABSTRACT:
SUBMITTER: Kwon JA
PROVIDER: S-EPMC1200295 | biostudies-literature | 2005 Sep
REPOSITORIES: biostudies-literature
Publications
Biological function of the vaccinia virus Z-DNA-binding protein E3L: gene transactivation and antiapoptotic activity in HeLa cells.
Kwon Jin-Ah JA Rich Alexander A
Proceedings of the National Academy of Sciences of the United States of America 20050826 36
The vaccinia virus (VV) E3L protein is essential for virulence and has anti-apoptotic activity. In mice, Z-DNA-binding activity of the N-terminal domain of E3L (Z alpha) is necessary for viral lethality. Here, we report that inhibition of hygromycin-B-induced apoptosis in HeLa cells depends on Z-DNA binding of the E3L Z alpha domain. Z-DNA-binding domains of other proteins are equally effective in blocking apoptosis. Using a transient reporter assay, we demonstrate transactivation of human IL-6, ...[more]
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