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Defined DNA/nanoparticle conjugates.

ABSTRACT: Glutathione monolayer-protected gold clusters were reacted by place exchange with 19- or 20-residue thiolated oligonucleotides. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleotides by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.

SUBMITTER: Ackerson CJ

PROVIDER: S-EPMC1200880 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature

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Defined DNA/nanoparticle conjugates.

Ackerson Christopher J CJ Sykes Michael T MT Kornberg Roger D RD

Proceedings of the National Academy of Sciences of the United States of America 20050909 38

Glutathione monolayer-protected gold clusters were reacted by place exchange with 19- or 20-residue thiolated oligonucleotides. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleotides by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content. ...[more]

PMID: 16155122

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