Defined DNA/nanoparticle conjugates.

ABSTRACT: Glutathione monolayer-protected gold clusters were reacted by place exchange with 19- or 20-residue thiolated oligonucleotides. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleotides by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.

SUBMITTER: Ackerson CJ 

PROVIDER: S-EPMC1200880 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature